• Immunofluorescence analysis of NIH/3T3 cells using LAMP1 Rabbit polyclonal antibody (STJ11105066) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using LAMP1 Rabbit polyclonal antibody (STJ11105066) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-LAMP1 antibody (208-363) (STJ11105066)

SKU:
STJ11105066

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LAMP1 (208-363) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Lamp1
Gene ID: 16783
Uniprot ID: LAMP1_MOUSE
Immunogen Region: 208-363
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 208-363 of mouse LAMP1 (NP_001304282.1).
Immunogen Sequence: PTVSKYNVTGNNGTCLLASM ALQLNITYLKKDNKTVTRAF NISPNDTSSGSCGINLVTLK VENKNRALELQFGMNASSSL FFLQGVRLNMTLPDALVPTF SISNHSLKALQATVGNSYKC NTEEHIFVSKMLSLNVFSVQ VQAFKVDSDRFGSVEE
Post Translational Modifications O- and N-glycosylated.some of the N-glycans attached to LAMP-1 are polylactosaminoglycans.
Function Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. Also plays an important role in NK-cells cytotoxicity. Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule. In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content. Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
Protein Name Lysosome-Associated Membrane Glycoprotein 1
Lamp-1
Lysosome-Associated Membrane Protein 1
120 Kda Lysosomal Membrane Glycoprotein
Cd107 Antigen-Like Family Member A
Lgp-120
Lysosomal Membrane Glycoprotein A
Lgp-A
P2b
Cd Antigen Cd107a
Database Links Reactome: R-MMU-6798695
Cellular Localisation Lysosome Membrane
Single-Pass Type I Membrane Protein
Endosome Membrane
Late Endosome Membrane
Cell Membrane
Cytolytic Granule Membrane
This Protein Shuttles Between Lysosomes
Endosomes
And The Plasma Membrane
Colocalizes With Osbpl1a At The Late Endosome
Alternative Antibody Names Anti-Lysosome-Associated Membrane Glycoprotein 1 antibody
Anti-Lamp-1 antibody
Anti-Lysosome-Associated Membrane Protein 1 antibody
Anti-120 Kda Lysosomal Membrane Glycoprotein antibody
Anti-Cd107 Antigen-Like Family Member A antibody
Anti-Lgp-120 antibody
Anti-Lysosomal Membrane Glycoprotein A antibody
Anti-Lgp-A antibody
Anti-P2b antibody
Anti-Cd Antigen Cd107a antibody
Anti-Lamp1 antibody
Anti-Lamp-1 antibody

Information sourced from Uniprot.org

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