• STJ72852 (0. 2µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Lamin B1-Isoform 1 antibody (Internal) (STJ72852)

SKU:
STJ72852-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Lamin B1-Isoform 1 (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 65kDa band observed in lysates of cell line Jurkat (calculated MW of 66.4kDa according to NP_005564.1). Recommended concentration: 0.1-0.3µg/ml.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: LMNB1
Gene ID: 4001
Uniprot ID: LMNB1_HUMAN
Immunogen Region: Internal
Accession Number: NP_005564.1
Specificity: This antibody is expected to recognize isoform 1 (NP_005564.1) only.
Immunogen Sequence: KESDLNGAQIKLRE
Function Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.
Protein Name Lamin-B1
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-2559584
Reactome: R-HSA-2980766
Reactome: R-HSA-2995383
Reactome: R-HSA-352238
Reactome: R-HSA-4419969
Reactome: R-HSA-8862803
Reactome: R-HSA-8950505
Reactome: R-HSA-9013405
Reactome: R-HSA-9035034
Cellular Localisation Nucleus Lamina
Alternative Antibody Names Anti-Lamin-B1 antibody
Anti-LMNB1 antibody
Anti-LMN2 antibody
Anti-LMNB antibody

Information sourced from Uniprot.org

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