| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Lamin B1-Isoform 1 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 65kDa band observed in lysates of cell line Jurkat (calculated MW of 66.4kDa according to NP_005564.1). Recommended concentration: 0.1-0.3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | LMNB1 |
| Gene ID: | 4001 |
| Uniprot ID: | LMNB1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_005564.1 |
| Specificity: | This antibody is expected to recognize isoform 1 (NP_005564.1) only. |
| Immunogen Sequence: | KESDLNGAQIKLRE |
| Function | Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively. |
| Protein Name | Lamin-B1 |
| Database Links | Reactome: R-HSA-1221632 Reactome: R-HSA-2559584 Reactome: R-HSA-2980766 Reactome: R-HSA-2995383 Reactome: R-HSA-352238 Reactome: R-HSA-4419969 Reactome: R-HSA-8862803 Reactome: R-HSA-8950505 Reactome: R-HSA-9013405 Reactome: R-HSA-9035034 |
| Cellular Localisation | Nucleus Lamina |
| Alternative Antibody Names | Anti-Lamin-B1 antibody Anti-LMNB1 antibody Anti-LMN2 antibody Anti-LMNB antibody |
Information sourced from Uniprot.org