• Immunofluorescence analysis of L929 cells using Laminin beta 1 Polyclonal Antibody (STJ11104364) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Laminin beta 1 antibody (STJ11104364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-LAMB1 antibody (1467-1786) (STJ11104364)

SKU:
STJ11104364

Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Laminin Subunit Beta-1 (1467-1786) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LAMB1
Gene ID: 3912
Uniprot ID: LAMB1_HUMAN
Immunogen Region: 1467-1786
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1467-1786 of human Laminin beta 1 (NP_002282.2).
Immunogen Sequence: EAKLRADEAKQSAEDILLKT NATKEKMDKSNEELRNLIKQ IRNFLTQDSADLDSIEAVAN EVLKMEMPSTPQQLQNLTED IRERVESLSQVEVILQHSAA DIARAEMLLEEAKRASKSAT DVKVTADMVKEALEEAEKAQ VAAEKAIKQADEDIQGTQNL LTSIESETAASEETLFNASQ RISELERNVEELKRKAAQNS GEAEYIEKVVYTVKQSAEDV KKTLDGELDEKYKKVENLI
Function Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of cerebral cortex. It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons. Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface.
Protein Name Laminin Subunit Beta-1
Laminin B1 Chain
Laminin-1 Subunit Beta
Laminin-10 Subunit Beta
Laminin-12 Subunit Beta
Laminin-2 Subunit Beta
Laminin-6 Subunit Beta
Laminin-8 Subunit Beta
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-3000157
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-373760
Reactome: R-HSA-381426
Reactome: R-HSA-8874081
Reactome: R-HSA-8957275
Reactome: R-HSA-9619665
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Major Component
Alternative Antibody Names Anti-Laminin Subunit Beta-1 antibody
Anti-Laminin B1 Chain antibody
Anti-Laminin-1 Subunit Beta antibody
Anti-Laminin-10 Subunit Beta antibody
Anti-Laminin-12 Subunit Beta antibody
Anti-Laminin-2 Subunit Beta antibody
Anti-Laminin-6 Subunit Beta antibody
Anti-Laminin-8 Subunit Beta antibody
Anti-LAMB1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance