| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Goat polyclonal antibody anti-LAG3/CD223 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:2000. WB: Approx 55kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 57.4kDa according to NP_002277.4). Recommended concentration: 0.3-1µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | LAG3 |
| Gene ID: | 3902 |
| Uniprot ID: | LAG3_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_002277.4 |
| Specificity: | This antibody is expected to recognize the extra-cellular domain. |
| Immunogen Sequence: | EDVSQAQAGTYTCH |
| Post Translational Modifications | Lymphocyte activation gene 3 protein: Proteolytically cleaved by ADAM10 and ADAM17 within the connecting peptide region, leading to release of Secreted lymphocyte activation gene 3 protein (sLAG-3). ADAM10 mediates constitutive cleavage, but cleavage increases following T-cell activation, whereas shedding by ADAM17 is induced by TCR signaling in a PRKCQ-dependent manner. |
| Function | Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as FGL1. FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function. Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1. Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells. Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function. Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation. Binds MHC class II (MHC-II).the precise role of MHC-II-binding is however unclear. Secreted lymphocyte activation gene 3 protein: May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response. |
| Protein Name | Lymphocyte Activation Gene 3 Protein Lag-3 Cd Antigen Cd223 Cleaved Into - Secreted Lymphocyte Activation Gene 3 Protein Slag-3 |
| Database Links | Reactome: R-HSA-2132295 |
| Cellular Localisation | Lymphocyte Activation Gene 3 Protein: Cell Membrane Single-Pass Type I Membrane Protein Secreted Lymphocyte Activation Gene 3 Protein: Secreted Produced Following Cleavage Of The Main Chain |
| Alternative Antibody Names | Anti-Lymphocyte Activation Gene 3 Protein antibody Anti-Lag-3 antibody Anti-Cd Antigen Cd223 Cleaved Into - Secreted Lymphocyte Activation Gene 3 Protein antibody Anti-Slag-3 antibody Anti-LAG3 antibody Anti-FDC antibody |
Information sourced from Uniprot.org
