• Western blot analysis of lysates from NIH/3T3 cells, using Lactic acid-Histone H3-K18 Rabbit monoclonal antibody (STJ11104234) at1:2000 dilution. NIH/3T3 cells were treated by Sodium lactate (100mM) for 24h. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Lactic acid-Histone H3-K18-K18 antibody [S4234RM] (STJ11104234)

SKU:
STJ11104234

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Lactic acid-Histone H3-K18-K18 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4234RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic lactylated peptide around K18 of human Lactic acid-Histone H3-K18 (NP_003520.1).
Immunogen Sequence: APRKQ
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Information sourced from Uniprot.org

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