• Immunohistochemistry analysis of paraffin-embedded human liver using L-Plastin/LCP1 Rabbit monoclonal antibody (STJ11103572) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using L-Plastin/LCP1 Rabbit monoclonal antibody (STJ11103572) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Raji cells, using L-Plastin/LCP1 antibody (STJ11103572) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-L-Plastin/LCP1 antibody (528-627) [S2MR] (STJ11103572)

SKU:
STJ11103572

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-L-Plastin/LCP1 (528-627) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LCP1
Gene ID: 3936
Uniprot ID: PLSL_HUMAN
Immunogen Region: 528-627
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 528-627 of human L-Plastin/LCP1 (P13796).
Immunogen Sequence: TLREAKKSSSISSFKDPKIS TSLPVLDLIDAIQPGSINYD LLKTENLNDDEKLNNAKYAI SMARKIGARVYALPEDLVEV NPKMVMTVFACLMGKGMKRV
Tissue Specificity Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T-lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.
Post Translational Modifications Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
Function Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
Protein Name Plastin-2
L-Plastin
Lc64p
Lymphocyte Cytosolic Protein 1
Lcp-1
Database Links Reactome: R-HSA-8950505
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Junction
Cell Projection
Ruffle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Relocalizes To The Immunological Synapse Between Peripheral Blood T-Lymphocytes And Antibody-Presenting Cells In Response To Costimulation Through Tcr/Cd3 And Cd2 Or Cd28
Associated With The Actin Cytoskeleton At Membrane Ruffles
Relocalizes To Actin-Rich Cell Projections Upon Serine Phosphorylation
Alternative Antibody Names Anti-Plastin-2 antibody
Anti-L-Plastin antibody
Anti-Lc64p antibody
Anti-Lymphocyte Cytosolic Protein 1 antibody
Anti-Lcp-1 antibody
Anti-LCP1 antibody
Anti-PLS2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance