• Western blot analysis of lysates from 293 cells, using KSR2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HeLa cells, using KSR2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-KSR2 antibody (671-720 aa) (STJ93862)

SKU:
STJ93862

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Kinase suppressor of Ras 2 (671-720 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KSR2
Gene ID: 283455
Uniprot ID: KSR2_HUMAN
Immunogen Region: 671-720 aa
Specificity: Ksr2 Polyclonal Antibody detects endogenous levels of Ksr2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human KSR2 at the amino acid range 671-720
Post Translational Modifications Phosphorylated on Ser-474 by MARK3.
Function Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1/MEK1-dependent allosteric activator of BRAF.upon binding to MAP2K1/MEK1, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1. Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
Protein Name Kinase Suppressor Of Ras 2
Hksr2
Database Links Reactome: R-HSA-5674135
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Kinase Suppressor Of Ras 2 antibody
Anti-Hksr2 antibody
Anti-KSR2 antibody

Information sourced from Uniprot.org

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