• SW480 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK8 (ABT536) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: SW480Predicted band size: 54kDaObserved band size: 50kDa
  • HepG2 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK8 (ABT536) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2Predicted band size: 54kDaObserved band size: 54kDa
  • Human appendix tissue was stained with Anti-Cytokeratin 8 (ABT536) Antibody
  • Human colon carcinomatissue was stained with Anti-Cytokeratin 8 (ABT536) Antibody
  • Human gastric adenocarcinoma tissue was stained with Anti-Cytokeratin 8 (ABT536) Antibody
  • Human liver tissue was stained with Anti-Cytokeratin 8 (ABT536) Antibody
  • Human lung adenocarcinoma tissue was stained with Anti-Cytokeratin 8 (ABT536) Antibody

Anti-KRT8 antibody (400-483 aa) [ABT536] (STJ197271)

SKU:
STJ197271

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Keratin, type II cytoskeletal 8 (400-483 aa) is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Monoclonal
Clone ID: ABT536
Conjugation: Unconjugated
Isotype: IgG2ak
Formulation: Liquid in PBS pH7.2, 0.03% Proclin 300, with stabilizing protein.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:200-400
WB 1:200-1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT8
Gene ID: 3856
Uniprot ID: K2C8_HUMAN
Immunogen Region: 400-483 aa
Specificity: The antibody can specifically recognize human CK8 protein, and shows no cross reaction with CK1, 4, 5, 6, 7, 10, 15, 16, 17, 18, 20.
Immunogen: Synthesized peptide derived from the human Cytokeratin 8 at the amino acid range 400-483
Post Translational Modifications Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Protein Name Keratin - Type Ii Cytoskeletal 8
Cytokeratin-8
Ck-8
Keratin-8
K8
Type-Ii Keratin Kb8
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Nucleus Matrix
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 8 antibody
Anti-Cytokeratin-8 antibody
Anti-Ck-8 antibody
Anti-Keratin-8 antibody
Anti-K8 antibody
Anti-Type-Ii Keratin Kb8 antibody
Anti-KRT8 antibody
Anti-CYK8 antibody

Information sourced from Uniprot.org

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