| Host: | Mouse |
| Applications: | IHC/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Keratin, type II cytoskeletal 5 (500-590 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Monoclonal |
| Clone ID : | ABT-CK5 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2ak |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range: | IHC-P 1:100-500 WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | KRT5 |
| Gene ID: | 3852 |
| Uniprot ID: | K2C5_HUMAN |
| Immunogen Region: | 500-590 aa |
| Specificity: | This antibody detects endogenous levels of human Cytokeratin 5. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section. The antibo |
| Immunogen: | Synthesized peptide derived from the human Cytokeratin 5 at the amino acid range 500-590 |
| Post Translational Modifications | Phosphorylated by CDK1, AURKB and Rho-kinase, phosphorylation is regulated by the cell cycle. Thr-24 phosphorylation, mediated by CDK1, peaks during prometaphase or metaphase cells with phosphorylated filamentous structures evident throughout the cytoplasm during early mitosis. CDK1 phosphorylates Thr-24 in mitotic cells at the site of injury. O-glycosylated. |
| Function | Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress. Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes. |
| Protein Name | Keratin - Type Ii Cytoskeletal 5 58 Kda Cytokeratin Cytokeratin-5 Ck-5 Keratin-5 K5 Type-Ii Keratin Kb5 |
| Database Links | Reactome: R-HSA-446107 Reactome: R-HSA-6805567 Reactome: R-HSA-6809371 Reactome: R-HSA-9725554 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Keratin - Type Ii Cytoskeletal 5 antibody Anti-58 Kda Cytokeratin antibody Anti-Cytokeratin-5 antibody Anti-Ck-5 antibody Anti-Keratin-5 antibody Anti-K5 antibody Anti-Type-Ii Keratin Kb5 antibody Anti-KRT5 antibody |
Information sourced from Uniprot.org