| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Cytokeratin 14 (264-457) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S7MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KRT14 |
| Gene ID: |
3861 |
| Uniprot ID: |
K1C14_HUMAN |
| Immunogen Region: |
264-457 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 264-457 of human Cytokeratin 14 (KRT14) (P02533). |
| Immunogen Sequence: |
VGGDVNVEMDAAPGVDLSRI LNEMRDQYEKMAEKNRKDAE EWFFTKTEELNREVATNSEL VQSGKSEISELRRTMQNLEI ELQSQLSMKASLENSLEETK GRYCMQLAQIQEMIGSVEEQ LAQLRCEMEQQNQEYKILLD VKTRLEQEIATYRRLLEGED AHLSSSQFSSGSQSSRDVTS SSRQIRTKVMDVHD |
| Tissue Specificity | Expressed in the corneal epithelium (at protein level). Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. |
| Post Translational Modifications | A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus. Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex. |
| Function | The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. |
| Protein Name | Keratin - Type I Cytoskeletal 14Cytokeratin-14Ck-14Keratin-14K14 |
| Database Links | Reactome: R-HSA-446107Reactome: R-HSA-6805567Reactome: R-HSA-6809371 |
| Cellular Localisation | CytoplasmNucleusExpressed In Both As A Filamentous Pattern |
| Alternative Antibody Names | Anti-Keratin - Type I Cytoskeletal 14 antibodyAnti-Cytokeratin-14 antibodyAnti-Ck-14 antibodyAnti-Keratin-14 antibodyAnti-K14 antibodyAnti-KRT14 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
