• Western blot analysis of various lysates using Cytokeratin 12 (KRT12) Rabbit polyclonal antibody (STJ116946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-KRT12 antibody (295-494) (STJ116946)

SKU:
STJ116946

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT12
Gene ID: 3859
Uniprot ID: K1C12_HUMAN
Immunogen Region: 295-494
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 295-494 of human Cytokeratin 12 (Cytokeratin 12 (KRT12) ) (NP_000214.1).
Immunogen Sequence: TRLLNDMRAQYETIAEQNRK DAEAWFIEKSGELRKEISTN TEQLQSSKSEVTDLRRAFQN LEIELQSQLAMKKSLEDSLA EAEGDYCAQLSQVQQLISNL EAQLLQVRADAERQNVDHQR LLNVKARLELEIETYRRLLD GEAQGDGLEESLFVTDSKSQ AQSTDSSKDPTKTRKIKTVV QEMVNGEVVSSQVQEIEELM
Tissue Specificity Expressed in the corneal epithelium (at protein level).
Function Involved in corneal epithelium organization, integrity and corneal keratin expression.
Protein Name Keratin - Type I Cytoskeletal 12
Cytokeratin-12
Ck-12
Keratin-12
K12
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 12 antibody
Anti-Cytokeratin-12 antibody
Anti-Ck-12 antibody
Anti-Keratin-12 antibody
Anti-K12 antibody
Anti-KRT12 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance