Anti-KNTC1 antibody (900-2209) (STJ115031)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal KNTC1 (900-2209) antibody for use in WB and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KNTC1 |
| Gene ID | 9735 |
| Uniprot ID | KNTC1_HUMAN |
| Immunogen Region | 900-2209 |
| Immunogen Sequence | IDLIDREQGEDCLLLLKSLP PAEAEKTAERVIIWARLALQ EEPDHSKEGKAWRMSVAKTS VDILKILCDIQKDNLQKKDE CEEMLKLFKEVASLQENFEV FLSFEDYSNSSLVADLREQH IKAHEVAQAKHKPGSTPEPI AAEVRSPSMESKLHRQALAL QMSKQELEAELTLRALKDGN IKTALKKCSDLFKYHCNADT G |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 900-1100 of human KNTC1 (NP_055523.1). |
Additional Info
| Tissue Specificity | High expression in testis. |
| Function | Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex. |
| Protein Name | Kinetochore-Associated Protein 1Rough Deal HomologHsrodRodHrod |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | CytoplasmNucleusChromosomeCentromereKinetochoreCytoskeletonSpindleDynamic Pattern Of Localization During The Cell CycleAt InterphaseUniformly Distributed Throughout The Cytoplasm And NucleusBy Prophase And Until Late Stages Of PrometaphaseA Fraction Of The Total Pool Is Concentrated At KinetochoresBy MetaphaseDetected At KinetochoresAlong Spindle Fibers And Most Prominently At The PolesBy Late Anaphase Until The End Of TelophaseNo Longer Detectable On Kinetochores Or Along Spindle FibersBut Still Present At The Spindle Poles |
| Alternative Antibody Names | Anti-Kinetochore-Associated Protein 1 antibodyAnti-Rough Deal Homolog antibodyAnti-Hsrod antibodyAnti-Rod antibodyAnti-Hrod antibodyAnti-KNTC1 antibodyAnti-KIAA0166 antibodyAnti-ROD antibody |
Information sourced from Uniprot.org