| Function | Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity. In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5C is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4. May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4. |
| Protein Name | Histone-Lysine N-Methyltransferase Kmt5cLysine N-Methyltransferase 5cLysine-Specific Methyltransferase 5cSuppressor Of Variegation 4-20 Homolog 2Su(Var4-20 Homolog 2Suv4-20h2Histone H4-N-Methyl-L-Lysine20 N-Methyltransferase Kmt5bHistone H4-Lysine20 N-Methyltransferase Kmt5b |
| Database Links | Reactome: R-HSA-3214841 |
| Cellular Localisation | NucleusChromosomeAssociated With Pericentric HeterochromatinCbx1 And Cbx5 Are Required For The Localization To Pericentric Heterochromatin |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Kmt5c antibodyAnti-Lysine N-Methyltransferase 5c antibodyAnti-Lysine-Specific Methyltransferase 5c antibodyAnti-Suppressor Of Variegation 4-20 Homolog 2 antibodyAnti-Su(Var4-20 Homolog 2 antibodyAnti-Suv4-20h2 antibodyAnti-Histone H4-N-Methyl-L-Lysine20 N-Methyltransferase Kmt5b antibodyAnti-Histone H4-Lysine20 N-Methyltransferase Kmt5b antibodyAnti-KMT5C antibodyAnti-SUV420H2 antibodyAnti-PP7130 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and KMT5C knockout (KO) 293T cells, using [KO Validated] KMT5C Rabbit polyclonal antibody (STJ118674) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/97562/380828/STJ118674_1__43548.1713148897.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and KMT5C knockout (KO) 293T cells, using [KO Validated] KMT5C Rabbit polyclonal antibody (STJ118674) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from Mouse thymus, using [KO Validated] KMT5C Rabbit polyclonal antibody (STJ118674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/97562/380829/STJ118674_2__10309.1713148898.jpg?c=1 "Western blot analysis of lysates from Mouse thymus, using [KO Validated] KMT5C Rabbit polyclonal antibody (STJ118674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of various lysates using [KO Validated] KMT5C Rabbit polyclonal antibody (STJ118674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/97562/380830/STJ118674_3__06334.1713148898.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] KMT5C Rabbit polyclonal antibody (STJ118674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and KMT5C knockout (KO) 293T cells, using [KO Validated] KMT5C Rabbit polyclonal antibody (STJ118674) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/97562/380828/STJ118674_1__43548.1713148897.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and KMT5C knockout (KO) 293T cells, using [KO Validated] KMT5C Rabbit polyclonal antibody (STJ118674) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
