• Immunoprecipitation analysis of 300 Mu g extracts of LNCaP cells using 3 Mu g KLK3 antibody (STJ24333). Western blot was performed from the immunoprecipitate using KLK3 antibody (STJ24333) at a dilution of 1:2000.
  • Immunofluorescence analysis of LnCap cells using KLK3 Rabbit polyclonal antibody (STJ24333) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of KLK3 in paraffin-embedded human prostate cancer using KLK3 Rabbit polyclonal antibody (STJ24333) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from LNCaP cells, using KLK3 Rabbit polyclonal antibody (STJ24333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-KLK3 antibody (30-261) (STJ24333)

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STJ24333

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KLK3 (30-261) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLK3
Gene ID: 354
Uniprot ID: KLK3_HUMAN
Immunogen Region: 30-261
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-261 of human KLK3 (NP_001639.1).
Immunogen Sequence: ECEKHSQPWQVLVASRGRAV CGGVLVHPQWVLTAAHCIRN KSVILLGRHSLFHPEDTGQV FQVSHSFPHPLYDMSLLKNR FLRPGDDSSHDLMLLRLSEP AELTDAVKVMDLPTQEPALG TTCYASGWGSIEPEEFLTPK KLQCVDLHVISNDVCAQVHP QKVTKFMLCAGRWTGGKSTC SGDSGGPLVCNGVLQGITSW GSEPCALPERPSLYTKVVHY RKWIKDTIVANP
Function Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Protein Name Prostate-Specific Antigen
Psa
Gamma-Seminoprotein
Seminin
Kallikrein-3
P-30 Antigen
Semenogelase
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-5625886
Cellular Localisation Secreted
Alternative Antibody Names Anti-Prostate-Specific Antigen antibody
Anti-Psa antibody
Anti-Gamma-Seminoprotein antibody
Anti-Seminin antibody
Anti-Kallikrein-3 antibody
Anti-P-30 Antigen antibody
Anti-Semenogelase antibody
Anti-KLK3 antibody
Anti-APS antibody

Information sourced from Uniprot.org

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