| Short Description : | Goat polyclonal antibody anti-KLF8/BKLF3 (N-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications. |
| Applications: | Pep-ELISA/IF/FC |
| Host: | Goat |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary testing showed bands at approx. 27+45kDa in HeLa nuclear cell lysate at a concentration of 1ug/ml (calculated Mwt. of 39.3kDa according to NP_009181.2 and 27.2kDa according |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | KLF8 |
| Gene ID: | 11279 |
| Uniprot ID: | KLF8_HUMAN |
| Accession Number: | NP_009181.2; NP_001152768.1 |
| Immunogen Region: | N-Term |
| Immunogen Sequence: | VDMDKLINNLEVQ |
| Specificity: | This antibody is expected to recognise isoform 1 (NP_009181.2) and isoform 2 (NP_001152768.1). |
| Post Translational Modifications | Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location. |
| Function | Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK). |
| Protein Name | Krueppel-Like Factor 8Basic Krueppel-Like Factor 3Zinc Finger Protein 741 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Krueppel-Like Factor 8 antibodyAnti-Basic Krueppel-Like Factor 3 antibodyAnti-Zinc Finger Protein 741 antibodyAnti-KLF8 antibodyAnti-BKLF3 antibodyAnti-ZNF741 antibody |
Information sourced from Uniprot.org
