• Immunohistochemistry analysis of paraffin-embedded human placenta using KLF5 antibody (STJ24327) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of HeLa, using KLF5 Rabbit polyclonal antibody (STJ24327) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-KLF5 antibody (1-250) (STJ24327)

SKU:
STJ24327

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KLF5 (1-250) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLF5
Gene ID: 688
Uniprot ID: KLF5_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KLF5 (NP_001721.2).
Immunogen Sequence: MATRVLSMSARLGPVPQPPA PQDEPVFAQLKPVLGAANPA RDAALFPGEELKHAHHRPQA QPAPAQAPQPAQPPATGPRL PPEDLVQTRCEMEKYLTPQL PPVPIIPEHKKYRRDSASVV DQFFTDTEGLPYSINMNVFL PDITHLRTGLYKSQRPCVTH IKTEPVAIFSHQSETTAPPP APTQALPEFTSIFSSHQTAA PEVNNIFIKQELPTPDLHLS VPTQQGHLYQLLNTPDLDM
Tissue Specificity Expressed only in testis and placenta.
Post Translational Modifications Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. Deubiquitinated by ATXN3L.
Function Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
Protein Name Krueppel-Like Factor 5
Basic Transcription Element-Binding Protein 2
Bte-Binding Protein 2
Colon Krueppel-Like Factor
Gc-Box-Binding Protein 2
Intestinal-Enriched Krueppel-Like Factor
Transcription Factor Bteb2
Database Links Reactome: R-HSA-381340
Reactome: R-HSA-9616222
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Krueppel-Like Factor 5 antibody
Anti-Basic Transcription Element-Binding Protein 2 antibody
Anti-Bte-Binding Protein 2 antibody
Anti-Colon Krueppel-Like Factor antibody
Anti-Gc-Box-Binding Protein 2 antibody
Anti-Intestinal-Enriched Krueppel-Like Factor antibody
Anti-Transcription Factor Bteb2 antibody
Anti-KLF5 antibody
Anti-BTEB2 antibody
Anti-CKLF antibody
Anti-IKLF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance