• Immunohistochemistry analysis of KLF12 in paraffin-embedded rat testis using KLF12 Rabbit polyclonal antibody (STJ11103322) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of KLF12 in paraffin-embedded mouse brain using KLF12 Rabbit polyclonal antibody (STJ11103322) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat liver using KLF12 Rabbit polyclonal antibody (STJ11103322) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-KLF12 antibody (220-310) (STJ11103322)

SKU:
STJ11103322

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KLF12 (220-310) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLF12
Gene ID: 11278
Uniprot ID: KLF12_HUMAN
Immunogen Region: 220-310
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-310 of human KLF12 (NP_009180.3).
Immunogen Sequence: GHGKAQMDPRGLSPRQSKSD SDDDDLPNVTLDSVNETGST ALSIARAVQEVHPSPVSRVR GNRMNNQKFPCSISPFSIES TRRQRRSESPD
Function Confers strong transcriptional repression to the AP-2-alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter.
Protein Name Krueppel-Like Factor 12
Transcriptional Repressor Ap-2rep
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Krueppel-Like Factor 12 antibody
Anti-Transcriptional Repressor Ap-2rep antibody
Anti-KLF12 antibody
Anti-AP2REP antibody
Anti-HSPC122 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance