| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Krueppel-Like Factor 1 (211-311) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KLF1 |
| Gene ID: |
10661 |
| Uniprot ID: |
KLF1_HUMAN |
| Immunogen Region: |
211-311 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 211-311 of human KLF1 (NP_006554.1). |
| Immunogen Sequence: |
QYQGHFQLFRGLQGPAPGPA TSPSFLSCLGPGTVGTGLGG TAEDPGVIAETAPSKRGRRS WARKRQAAHTCAHPGCGKSY TKSSHLKAHLRTHTGEKPYA C |
| Tissue Specificity | Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines. |
| Post Translational Modifications | Acetylated.can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity. Sumoylated.sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex. Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity. |
| Function | Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation. |
| Protein Name | Krueppel-Like Factor 1Erythroid Krueppel-Like Transcription FactorEklf |
| Cellular Localisation | NucleusColocalizes With Sumo1 In Nuclear Speckles |
| Alternative Antibody Names | Anti-Krueppel-Like Factor 1 antibodyAnti-Erythroid Krueppel-Like Transcription Factor antibodyAnti-Eklf antibodyAnti-KLF1 antibodyAnti-EKLF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
