• Immunofluorescence analysis of U-2 OS cells using KIFC1 Rabbit polyclonal antibody (STJ116198) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of KIFC1 in paraffin-embedded mouse testis using KIFC1 Rabbit polyclonal antibody (STJ116198) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of KIFC1 in paraffin-embedded human colon carcinoma using KIFC1 Rabbit polyclonal antibody (STJ116198) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of KIFC1 in paraffin-embedded rat testis using KIFC1 Rabbit polyclonal antibody (STJ116198) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using KIFC1 Rabbit polyclonal antibody (STJ116198) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-KIFC1 antibody (1-250) (STJ116198)

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STJ116198

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KIFC1 (1-250) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIFC1
Gene ID: 3833
Uniprot ID: KIFC1_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KIFC1 (NP_002254.2).
Immunogen Sequence: MDPQRSPLLEVKGNIELKRP LIKAPSQLPLSGSRLKRRPD QMEDGLEPEKKRTRGLGATT KITTSHPRVPSLTTVPQTQG QTTAQKVSKKTGPRCSTAIA TGLKNQKPVPAVPVQKSGTS GVPPMAGGKKPSKRPAWDLK GQLCDLNAELKRCRERTQTL DQENQQLQDQLRDAQQQVKA LGTERTTLEGHLAKVQAQAE QGQQELKNLRACVLELEERL STQEGLVQELQKKQVELQE
Function Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. Regulates cilium formation and structure.
Protein Name Kinesin-Like Protein Kifc1
Kinesin-Like Protein 2
Kinesin-Related Protein Hset
Database Links Reactome: R-HSA-6811434
Reactome: R-HSA-983189
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Early Endosome
Associated With Nucleus During Interphase
Centrosomes In Early And Spindle In Later Mitosis
Alternative Antibody Names Anti-Kinesin-Like Protein Kifc1 antibody
Anti-Kinesin-Like Protein 2 antibody
Anti-Kinesin-Related Protein Hset antibody
Anti-KIFC1 antibody
Anti-HSET antibody
Anti-KNSL2 antibody

Information sourced from Uniprot.org

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