• Western blot analysis of extracts of various cell lines, using KIF3A antibody (STJ28722) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using KIF3A rabbit polyclonal antibody (STJ28722) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of MCF7 cells using KIF3A antibody (STJ28722). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells, using 3 Mu g KIF3A antibody (STJ28722). Western blot was performed from the immunoprecipitate using KIF3A antibody (STJ28722) at a dilution of 1:1000.

Anti-KIF3A antibody (430-699) (STJ28722)

SKU:
STJ28722

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KIF3A (430-699) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:500
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIF3A
Gene ID: 11127
Uniprot ID: KIF3A_HUMAN
Immunogen Region: 430-699
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 430-699 of human KIF3A (NP_008985.3).
Immunogen Sequence: RKALETKLDMEEEERNKARA ELEKREKDLLKAQQEHQSLL EKLSALEKKVIVGGVDLLAK AEEQEKLLEESNMELEERRK RAEQLRRELEEKEQERLDIE EKYTSLQEEAQGKTKKLKKV WTMLMAAKSEMADLQQEHQR EIEGLLENIRQLSRELRLQM LIIDNFIPRDYQEMIENYVH WNEDIGEWQLKCVAYTGNNM RKQTPVPDKKEKDPFEVDLS HVYLAYTEESLRQSLMKLE
Function Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitment of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole.
Protein Name Kinesin-Like Protein Kif3a
Microtubule Plus End-Directed Kinesin Motor 3a
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-2132295
Reactome: R-HSA-5610787
Reactome: R-HSA-5620924
Reactome: R-HSA-5635838
Reactome: R-HSA-6811434
Reactome: R-HSA-983189
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Cilium
Microtubule Organizing Center
Centrosome
Centriole
Localizes To The Subdistal Appendage Region Of The Centriole
Alternative Antibody Names Anti-Kinesin-Like Protein Kif3a antibody
Anti-Microtubule Plus End-Directed Kinesin Motor 3a antibody
Anti-KIF3A antibody
Anti-KIF3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance