• Western blot analysis of extracts of various cell lines, using EG5/EG5/KIF11 antibody (STJ110216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of U2OS cells using EG5/KIF11 Rabbit polyclonal antibody (STJ110216) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using EG5/EG5/KIF11 antibody (STJ110216) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using EG5/KIF11 Rabbit polyclonal antibody (STJ110216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-KIF11 antibody (797-1056) (STJ110216)

SKU:
STJ110216

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KIF11 (797-1056) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:100
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIF11
Gene ID: 3832
Uniprot ID: KIF11_HUMAN
Immunogen Region: 797-1056
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 797-1056 of human EG5/EG5/KIF11 (NP_004514.2).
Immunogen Sequence: SVKHSDKLNGNLEKISQETE QRCESLNTRTVYFSEQWVSS LNEREQELHNLLEVVSQCCE ASSSDITEKSDGRKAAHEKQ HNIFLDQMTIDEDKLIAQNL ELNETIKIGLTKLNCFLEQD LKLDIPTGTTPQRKSYLYPS TLVRTEPREHLLDQLKRKQP ELLMMLNCSENNKEETIPDV DVEEAVLGQYTEEPLSQEPS VDAGVDCSSIGGVPFFQHKK SHGKDKENRGINTLERSKV
Post Translational Modifications Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis.phosphorylation is required for mitotic function.
Function Motor protein required for establishing a bipolar spindle during mitosis. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.
Protein Name Kinesin-Like Protein Kif11
Kinesin-Like Protein 1
Kinesin-Like Spindle Protein Hksp
Kinesin-Related Motor Protein Eg5
Thyroid Receptor-Interacting Protein 5
Tr-Interacting Protein 5
Trip-5
Database Links Reactome: R-HSA-2132295
Reactome: R-HSA-6811434
Reactome: R-HSA-983189
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle Pole
Alternative Antibody Names Anti-Kinesin-Like Protein Kif11 antibody
Anti-Kinesin-Like Protein 1 antibody
Anti-Kinesin-Like Spindle Protein Hksp antibody
Anti-Kinesin-Related Motor Protein Eg5 antibody
Anti-Thyroid Receptor-Interacting Protein 5 antibody
Anti-Tr-Interacting Protein 5 antibody
Anti-Trip-5 antibody
Anti-KIF11 antibody
Anti-EG5 antibody
Anti-KNSL1 antibody
Anti-TRIP5 antibody

Information sourced from Uniprot.org

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