| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PCLAF |
| Gene ID: |
9768 |
| Uniprot ID: |
PAF15_HUMAN |
| Immunogen Region: |
39-111 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 39-111 of human KIAA0101 (NP_055551.1). |
| Immunogen Sequence: |
SSRKAENKYAGGNPVCVRPT PKWQKGIGEFFRLSPKDSEK ENQIPEEAGSSGLGKAKRKA CPLQPDHTNDEKE |
| Tissue Specificity | Expressed predominantly in liver, pancreas and placenta. Not detected in heart or brain. Highly expressed in a number of tumors, especially esophageal tumors, in anaplastic thyroid carcinomas, adrenocortical carcinomas, and in non-small-cell lung cancer lines. |
| Post Translational Modifications | Monoubiquitinated at Lys-15 and Lys-24 during normal S phase, promoting its association with PCNA. Also diubiquitinated at these 2 sites. Following DNA damage, monoubiquitin chains at Lys-15 and Lys-24 are probably extended, leading to disrupt the interaction with PCNA. Polyubiquitinated by the APC/C complex at the mitotic exit, leading to its degradation by the proteasome. |
| Function | PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number. |
| Protein Name | Pcna-Associated FactorHepatitis C Virus Ns5a-Transactivated Protein 9Hcv Ns5a-Transactivated Protein 9Overexpressed In Anaplastic Thyroid Carcinoma 1Oeatc-1Pcna-Associated Factor Of 15 KdaPaf15P15pafPcna-Clamp-Associated Factor |
| Database Links | Reactome: R-HSA-5656169 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionFollowing Dna DamageLocalizes To Dna Damage SitesColocalizes With Centrosomes In Perinuclear Region |
| Alternative Antibody Names | Anti-Pcna-Associated Factor antibodyAnti-Hepatitis C Virus Ns5a-Transactivated Protein 9 antibodyAnti-Hcv Ns5a-Transactivated Protein 9 antibodyAnti-Overexpressed In Anaplastic Thyroid Carcinoma 1 antibodyAnti-Oeatc-1 antibodyAnti-Pcna-Associated Factor Of 15 Kda antibodyAnti-Paf15 antibodyAnti-P15paf antibodyAnti-Pcna-Clamp-Associated Factor antibodyAnti-PCLAF antibodyAnti-KIAA0101 antibodyAnti-NS5ATP9 antibodyAnti-PAF antibodyAnti-L5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
