| Host: | Mouse |
| Applications: | FC/WB/IF/IHC/IP |
| Reactivity: | R.Temporaria/X.laevis/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Keratin 8 is suitable for use in Flow Cytometry, Western Blot, Immunofluorescence, Immunohistochemistry and Immunoprecipitation research applications. |
| Clonality : | Monoclonal |
| Clone ID : | E2 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1k |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Purification: | Affinity purified from tissue culture. |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: | Flow Cytometry (1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunoblotting (1-2 µg/ml). Immunofluorescence (1-2 µg/ml). Immunohistology (1-2 µg/ml for 30 mi |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: | Krt18 |
| Gene ID: | 294853 |
| Uniprot ID: | K1C18_RAT |
| Immunogen: | A BALB/c mouse was immunized with an intermediate filament preparation of rat colon mucosa. |
| Post Translational Modifications | Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-231 by either caspase-3, caspase-6 or caspase-7. Dephosphorylated by ethanol. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. |
| Function | When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. |
| Protein Name | Keratin - Type I Cytoskeletal 18Cytokeratin-18Ck-18Keratin-18K18 |
| Database Links | Reactome: R-RNO-6805567Reactome: -RNO-6809371 |
| Cellular Localisation | Nucleus MatrixCytoplasmPerinuclear RegionNucleusNucleolus |
| Alternative Antibody Names | Anti-Keratin - Type I Cytoskeletal 18 antibodyAnti-Cytokeratin-18 antibodyAnti-Ck-18 antibodyAnti-Keratin-18 antibodyAnti-K18 antibodyAnti-Krt18 antibodyAnti-Krt1-18 antibody |
Information sourced from Uniprot.org