| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KDM8 (147-416) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KDM8 |
| Gene ID: |
79831 |
| Uniprot ID: |
KDM8_HUMAN |
| Immunogen Region: |
147-416 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 147-416 of human KDM8 (NP_079049.2). |
| Immunogen Sequence: |
THLPGKRPARGSLPEQPCTK KARADHGLIPDVKLEKTVPR LHRPSLQHFREQFLVPGRPV ILKGVADHWPCMQKWSLEYI QEIAGCRTVPVEVGSRYTDE EWSQTLMTVNEFISKYIVNE PRDVGYLAQHQLFDQIPELK QDISIPDYCSLGDGEEEEIT INAWFGPQGTISPLHQDPQQ NFLVQVMGRKYIRLYSPQES GALYPHDTHLLHNTSQVDVE NPDLEKFPKFAKAPFLSCI |
| Tissue Specificity | Weakly expressed in most cells. Highly expressed in breast cancer cells. Expressed in embryonic stem cells. |
| Function | Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monooxygenase. Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation. Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity. Upon DNA damage, cleaves the N-terminal tail of histone H3 at monomethylated lysine residues, preferably at monomethylated 'Lys-9' (H3K9me1). The histone variant H3F3A is the major target for cleavage. Additionnally, acts as Fe(2+) and 2-oxoglutarate-dependent monooxygenase, catalyzing (R)-stereospecific hydroxylation at C-3 of 'Arg-137' of RPS6 and 'Arg-141' of RCCD1, but the biological significance of this activity remains to be established. Regulates mitosis through different mechanisms: Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with RCCD1. Possibly together with RCCD1, is involved in proper mitotic spindle organization and chromosome segregation. Negatively regulates cell cycle repressor CDKN1A/p21, which controls G1/S phase transition. Required for G2/M phase cell cycle progression. Regulates expression of CCNA1/cyclin-A1, leading to cancer cell proliferation. Also, plays a role in regulating alpha-tubulin acetylation and cytoskeletal microtubule stability involved in epithelial to mesenchymal transition. Regulates the circadian gene expression in the liver. Represses the transcriptional activator activity of the CLOCK-BMAL1 heterodimer in a catalytically-independent manner. Negatively regulates the protein stability and function of CRY1.required for AMPK-FBXL3-induced CRY1 degradation. |
| Protein Name | Bifunctional Peptidase And Arginyl-Hydroxylase Jmjd5Jmjc Domain-Containing Protein 5Jumonji C Domain-Containing Protein 5L-Arginine3r-Hydroxylase Kdm8 |
| Cellular Localisation | NucleusChromosomeColocalizes With Trimethylated 'Lys-9' Of Histone H3 (H3k9me3) |
| Alternative Antibody Names | Anti-Bifunctional Peptidase And Arginyl-Hydroxylase Jmjd5 antibodyAnti-Jmjc Domain-Containing Protein 5 antibodyAnti-Jumonji C Domain-Containing Protein 5 antibodyAnti-L-Arginine antibodyAnti-3r-Hydroxylase Kdm8 antibodyAnti-KDM8 antibodyAnti-JMJD5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
