• Immunohistochemistry analysis of paraffin-embedded colon cancer tissues (left) and human larynx cancer tissues (right) with DAB staining using JMJD2A monoclonal antibody.
  • Immunofluorescence analysis of NTERA-2 cells using JMJD2A monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western blot analysis using JMJD2A monoclonal antibody against HEK293 (1) and JMJD2A-hIgGFc transfected HEK293 (2) cell lysate.

Anti-KDM4A antibody [5H1] (STJ98191)

SKU:
STJ98191

£144.00 - £348.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Lysine-specific demethylase 4A demethylase 4A demethylase 4A is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Monoclonal
Clone ID: 5H1
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KDM4A
Gene ID: 9682
Uniprot ID: KDM4A_HUMAN
Specificity: JMJD2A Monoclonal Antibody detects endogenous levels of JMJD2A protein.
Immunogen: Purified recombinant fragment of human JMJD2A expressed in E. Coli.
Post Translational Modifications (Microbial infection) SUMOylated by human herpesvirus 8 E3 SUMO-protein ligase K-bZIP/K8 at Lys-471.thereby modulating the chromatin binding and histone demethylase activity of KDM4A. Ubiquitinated by RNF8 and RNF168 following DNA damage, leading to its degradation. Degradation promotes accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited.
Function Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain.
Protein Name Lysine-Specific Demethylase 4a
Jmjc Domain-Containing Histone Demethylation Protein 3a
Jumonji Domain-Containing Protein 2a
Histone H3-Trimethyl-L-Lysine(36 Demethylase 4a
Histone H3-Trimethyl-L-Lysine(9 Demethylase 4a
Database Links Reactome: R-HSA-3214842
Reactome: R-HSA-5693565
Reactome: R-HSA-9029569
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Lysine-Specific Demethylase 4a antibody
Anti-Jmjc Domain-Containing Histone Demethylation Protein 3a antibody
Anti-Jumonji Domain-Containing Protein 2a antibody
Anti-Histone H3-Trimethyl-L-Lysine(36 Demethylase 4a antibody
Anti-Histone H3-Trimethyl-L-Lysine(9 Demethylase 4a antibody
Anti-KDM4A antibody
Anti-JHDM3A antibody
Anti-JMJD2 antibody
Anti-JMJD2A antibody
Anti-KIAA0677 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance