Anti-KCNQ2 antibody (466-665) (STJ111121)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal KCNQ2 (466-665) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KCNQ2 |
| Gene ID | 3785 |
| Uniprot ID | KCNQ2_HUMAN |
| Immunogen Region | 466-665 |
| Immunogen Sequence | SPSADQSLEDSPSKVPKSWS FGDRSRARQAFRIKGAASRQ NSEEASLPGEDIVDDKSCPC EFVTEDLTPGLKVSIRAVCV MRFLVSKRKFKESLRPYDVM DVIEQYSAGHLDMLSRIKSL QSRVDQIVGRGPAITDKDRT KGPAEAELPEDPSMMGRLGK VEKQVLSMEKKLDFLVNIYM QRMGIPPTETEAYFGAKEPE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 466-665 of human KCNQ2 (NP_742105.1). |
Additional Info
| Tissue Specificity | In adult and fetal brain. Highly expressed in areas containing neuronal cell bodies, low in spinal cord and corpus callosum. Isoform 2 is preferentially expressed in differentiated neurons. Isoform 6 is prominent in fetal brain, undifferentiated neuroblastoma cells and brain tumors. |
| Post Translational Modifications | KCNQ2/KCNQ3 heteromeric current can be increased by intracellular cyclic AMP, an effect that depends on phosphorylation of Ser-52 in the N-terminal region. KCNQ2/KCNQ3 are ubiquitinated by NEDD4L. Ubiquitination leads to protein degradation (Probable). Degradation induced by NEDD4L is inhibited by USP36. |
| Function | Pore-forming subunit of the voltage-gated potassium (Kv) M-channel which is responsible for the M-current, a key controller of neuronal excitability. M-channel is composed of pore-forming subunits KCNQ2 and KCNQ3 assembled as heterotetramers. The native M-current has a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. KCNQ2-KCNQ3 M-channel is selectively permeable in vitro to other cations besides potassium, in decreasing order of affinity K(+) > Rb(+) > Cs(+) > Na(+). M-channel association with SLC5A3/SMIT1 alters channel ion selectivity, increasing Na(+) and Cs(+) permeation relative to K(+). Suppressed by activation of the muscarinic acetylcholine receptor CHRM1. |
| Protein Name | Potassium Voltage-Gated Channel Subfamily Kqt Member 2Kqt-Like 2Neuroblastoma-Specific Potassium Channel Subunit Alpha Kvlqt2Voltage-Gated Potassium Channel Subunit Kv7.2 |
| Database Links | Reactome: R-HSA-1296072Reactome: R-HSA-445095 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Potassium Voltage-Gated Channel Subfamily Kqt Member 2 antibodyAnti-Kqt-Like 2 antibodyAnti-Neuroblastoma-Specific Potassium Channel Subunit Alpha Kvlqt2 antibodyAnti-Voltage-Gated Potassium Channel Subunit Kv7.2 antibodyAnti-KCNQ2 antibody |
Information sourced from Uniprot.org