| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KCNQ1 (250-549) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KCNQ1 |
| Gene ID: |
3784 |
| Uniprot ID: |
KCNQ1_HUMAN |
| Immunogen Region: |
250-549 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 250-549 of human KCNQ1 (NP_861463.1). |
| Immunogen Sequence: |
TAWRCYAAENPDSSTWKIYI RKAPRSHTLLSPSPKPKKSV VVKKKKFKLDKDNGVTPGEK MLTVPHITCDPPEERRLDHF SVDGYDSSVRKSPTLLEVSM PHFMRTNSFAEDLDLEGETL LTPITHISQLREHHRATIKV IRRMQYFVAKKKFQQARKPY DVRDVIEQYSQGHLNLMVRI KELQRRLDQSIGKPSLFISV SEKSKDRGSNTIGARLNRVE DKVTQLDQRLALITDMLHQ |
| Tissue Specificity | Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries. |
| Post Translational Modifications | Phosphorylation at Ser-27 by PKA.increases delayed rectifier potassium channel activity of the KCNQ1-KCNE1 complex through a macromolecular complex that includes PKA, PP1, and the targeting protein AKAP9. Ubiquitinated by NEDD4L.promotes internalization. The ubiquitinylated form is internalized through a clathrin-mediated endocytosis by interacting with AP2M1 and is recycled back to the cell membrane via RAB4A and RAB11A. Deubiquitinated by USP2.counteracts the NEDD4L-specific down-regulation of I(Ks) and restores the membrane localization. |
| Function | Potassium channel that plays an important role in a number of tissues, including heart, inner ear, stomach and colon. Associates with KCNE beta subunits that modulates current kinetics. Induces a voltage-dependent current by rapidly activating and slowly deactivating potassium-selective outward current. Promotes also a delayed voltage activated potassium current showing outward rectification characteristic. During beta-adrenergic receptor stimulation participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current. When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions. This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents. During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion. Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion. When associated with KCNE2, forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current. When associated with KCNE4, inhibits voltage-gated potassium channel activity. When associated with KCNE5, this complex only conducts current upon strong and continued depolarization. Also forms a heterotetramer with KCNQ5.has a voltage-gated potassium channel activity. Binds with phosphatidylinositol 4,5-bisphosphate. KCNQ1-KCNE2 channel associates with Na(+)-coupled myo-inositol symporter in the apical membrane of choroid plexus epithelium and regulates the myo-inositol gradient between blood and cerebrospinal fluid with an impact on neuron excitability. Isoform 2: Non-functional alone but modulatory when coexpressed with the full-length isoform 1. |
| Protein Name | Potassium Voltage-Gated Channel Subfamily Kqt Member 1Iks Producing Slow Voltage-Gated Potassium Channel Subunit Alpha Kvlqt1Kqt-Like 1Voltage-Gated Potassium Channel Subunit Kv7.1 |
| Database Links | Reactome: R-HSA-1296072Reactome: R-HSA-5576890Reactome: R-HSA-5576893 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCytoplasmic Vesicle MembraneEarly EndosomeMembrane RaftEndoplasmic ReticulumBasolateral Cell MembraneApical Cell MembraneColocalized With Kcne3 At The Plasma MembraneUpon 17beta-Oestradiol TreatmentColocalizes With Rab5a At Early EndosomeHeterotetramer With Kcnq5 Is Highly Retained At The Endoplasmic Reticulum And Is Localized Outside Of Lipid Raft MicrodomainsDuring The Early Stages Of Epithelial Cell Polarization Induced By The Calcium SwitchIt Is Removed From The Plasma Membrane To The Endoplasmic ReticulumWhere It Is RetainedAnd Redistributed To The Basolateral Cell Surface In A Pi3k-Dependent Manner At A Later StageColocalizes With Slc5a3 At The Apical Membrane Of Choroid Plexus Epithelium |
| Alternative Antibody Names | Anti-Potassium Voltage-Gated Channel Subfamily Kqt Member 1 antibodyAnti-Iks Producing Slow Voltage-Gated Potassium Channel Subunit Alpha Kvlqt1 antibodyAnti-Kqt-Like 1 antibodyAnti-Voltage-Gated Potassium Channel Subunit Kv7.1 antibodyAnti-KCNQ1 antibodyAnti-KCNA8 antibodyAnti-KCNA9 antibodyAnti-KVLQT1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
