| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human/Mouse/Rat/Dog/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-KCNN2 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 70kDa and 23kDa bands observed in Human Liver lysates (calculated MW of 63.8kDa according to NP_067627.2 and of 26.3kDa according to NP_740721.1). Recommended concentration: 0.3 |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
KCNN2 |
| Gene ID: |
3781 |
| Uniprot ID: |
KCNN2_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_067627.2; NP_740721.1 |
| Specificity: |
This antibody is expected to recognize both isoforms (NP_067627.2; NP_740721.1). |
| Immunogen Sequence: |
ESYDKHVTYNAER |
| Function | Small conductance calcium-activated potassium channel that mediates the voltage-independent transmembrane transfer of potassium across the cell membrane through a constitutive interaction with calmodulin which binds the intracellular calcium allowing its opening. The current is characterized by a voltage-independent activation, an intracellular calcium concentration increase-dependent activation and a single-channel conductance of about 3 picosiemens. Also presents an inwardly rectifying current, thus reducing its already small outward conductance of potassium ions, which is particularly the case when the membrane potential displays positive values, above + 20 mV. The inward rectification could be due to a blockade of the outward current by intracellular divalent cations such as calcium and magnesium and could also be due to an intrinsic property of the channel pore, independent of intracellular divalent ions. There are three positively charged amino acids in the S6 transmembrane domain, close to the pore, that collectively control the conductance and rectification through an electrostatic mechanism. Additionally, electrostatic contributions from these residues also play an important role in determining the intrinsic open probability of the channel in the absence of calcium, affecting the apparent calcium affinity for activation. Forms an heteromeric complex with calmodulin, which is constitutively associated in a calcium-independent manner. Channel opening is triggered when calcium binds the calmodulin resulting in a rotary movement leading to the formation of the dimeric complex to open the gate. Plays a role in the repolarization phase of cardiac action potential. |
| Protein Name | Small Conductance Calcium-Activated Potassium Channel Protein 2Sk2Skca 2Skca2Kca2.2 |
| Database Links | Reactome: R-HSA-1296052Reactome: R-HSA-9667769 |
| Cellular Localisation | MembraneMulti-Pass Membrane ProteinCytoplasmMyofibrilSarcomereZ Line |
| Alternative Antibody Names | Anti-Small Conductance Calcium-Activated Potassium Channel Protein 2 antibodyAnti-Sk2 antibodyAnti-Skca 2 antibodyAnti-Skca2 antibodyAnti-Kca2.2 antibodyAnti-KCNN2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
