| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KCNN2 (2-50 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Kcnn2 |
| Gene ID: |
140492 |
| Uniprot ID: |
KCNN2_MOUSE |
| Immunogen Region: |
2-50 aa |
| Specificity: |
Specific for KCNN2. |
| Immunogen: |
A synthetic peptide from aa region 2-50 of mouse KCNN2 (SK2) conjugated to an immunogenic carrier protein was used as the antigen. |
| Function | Small conductance calcium-activated potassium channel that mediates the voltage-independent transmembrane transfer of potassium across the cell membrane through a constitutive interaction with calmodulin which binds the intracellular calcium allowing its opening. The current is characterized by a voltage-independent activation, an intracellular calcium concentration increase-dependent activation and a single-channel conductance of about 3 picosiemens. Also presents an inwardly rectifying current, thus reducing its already small outward conductance of potassium ions, which is particularly the case when the membrane potential displays positive values, above + 20 mV. The inward rectification could be due to a blockade of the outward current by intracellular divalent cations such as calcium and magnesium and could also be due to an intrinsic property of the channel pore, independent of intracellular divalent ions. There are three positively charged amino acids in the S6 transmembrane domain, close to the pore, that collectively control the conductance and rectification through an electrostatic mechanism. Additionally, electrostatic contributions from these residues also play an important role in determining the intrinsic open probability of the channel in the absence of calcium, affecting the apparent calcium affinity for activation. Forms an heteromeric complex with calmodulin, which is constitutively associated in a calcium-independent manner. Channel opening is triggered when calcium binds the calmodulin resulting in a rotary movement leading to the formation of the dimeric complex to open the gate. Plays a role in the repolarization phase of cardiac action potential. |
| Protein Name | Small Conductance Calcium-Activated Potassium Channel Protein 2Sk2Skca 2Skca2Kca2.2 |
| Database Links | Reactome: R-MMU-1296052 |
| Cellular Localisation | MembraneMulti-Pass Membrane ProteinCytoplasmMyofibrilSarcomereZ Line |
| Alternative Antibody Names | Anti-Small Conductance Calcium-Activated Potassium Channel Protein 2 antibodyAnti-Sk2 antibodyAnti-Skca 2 antibodyAnti-Skca2 antibodyAnti-Kca2.2 antibodyAnti-Kcnn2 antibodyAnti-Sk2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
