Anti-KCNK18 antibody (N-Term) (STJ13101290)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the n-terminal region of mouse KCNK18 conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-KCNK18 (N-Term) for use in IHC and WB in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Kcnk18 |
| Gene ID | 332396 |
| Uniprot ID | KCNKI_MOUSE |
| Immunogen | A synthetic peptide from the n-terminal region of mouse KCNK18 conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | N-Term |
| Specificity | Specific for KCNK18. |
Additional Info
| Post Translational Modifications | Phosphorylation of Ser-264 is required for the binding of 14-3-3eta/YWHAH. Calcineurin-mediated dephosphorylation of Ser-276 enhances channel activity. N-glycosylated. |
| Function | K(+) channel that conducts outward and inward rectifying currents at depolarized and hyperpolarized membrane potentials, respectively. The outward rectifying currents are voltage-dependent, coupled to K(+) electrochemical gradient across the membrane, whereas the inward currents can be induced in response to activation of Ca(2+)-mobilizing receptors. Homo- and heterodimerizes to form functional channels with distinct regulatory and gating properties. In trigeminal ganglia sensory neurons, the heterodimers of KCNK18/TRESK and KCNK2/TREK-1 or KCNK10/TREK-2 inhibit neuronal firing and neurogenic inflammation by stabilizing the resting membrane potential at K(+) equilibrium potential as well as by regulating the threshold of action potentials and the spike frequency. In thymocytes, conducts K(+) currents upon T cell receptor (TCR) signaling leading to sustained Ca(2+) influx and NF-kappa-B activation, FOXP3 transcription and positive selection of regulatory T cell (Treg) progenitor subsets. Appears to mediate the analgesics effects of hydroxy-alpha-sanshool, a metabolite naturally present in Schezuan pepper and other Xanthoxylum plants. |
| Protein Name | Potassium Channel Subfamily K Member 18Two-Pore-Domain Potassium Channel Tresk |
| Database Links | Reactome: R-MMU-1299344Reactome: -MMU-5576886 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Potassium Channel Subfamily K Member 18 antibodyAnti-Two-Pore-Domain Potassium Channel Tresk antibodyAnti-Kcnk18 antibodyAnti-Tresk-2 antibodyAnti-Tresk2 antibody |
Information sourced from Uniprot.org