Anti-KCNK16 antibody (10-60 aa) (STJ13101288)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 10-60 of human KCNK16 conjugated to blue carrier protein was used as the antigen. The antigen shares about 95% identity with rat and mouse sequences.
General Information
| Short Description | Rabbit polyclonal anti-KCNK16 (10-60 aa) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | KCNK16 |
| Gene ID | 83795 |
| Uniprot ID | KCNKG_HUMAN |
| Immunogen | A synthetic peptide from aa region 10-60 of human KCNK16 conjugated to blue carrier protein was used as the antigen. The antigen shares about 95% identity with rat and mouse sequences. |
| Immunogen Region | 10-60 aa |
| Specificity | Specific for KCNK16. |
Additional Info
| Function | K(+) channel that conducts voltage-dependent outward rectifying currents upon membrane depolarization. Voltage sensing is coupled to K(+) electrochemical gradient in an 'ion flux gating' mode where outward but not inward ion flow opens the gate. Homo- and heterodimerizes to form functional channels with distinct regulatory and gating properties. In pancreatic islets, conducts K(+) countercurrents for Ca(2+) release from the endoplasmic reticulum (ER) and regulates the frequency and duration of cytosolic Ca(2+) oscillations coupled to secretion of pancreatic hormones. In pancreatic beta cells, drives ER Ca(2+) efflux, which in turn activates Ca(2+)-dependent plasma membrane K(+) slow currents and cytosolic Ca(2+) influx, overall contributing to synchronous cytosolic Ca(2+) oscillations. Limits glucose-induced cytosolic Ca(2+) oscillations coupled to second-phase INS secretion. Contributes to beta cell adaptation to acute inflammation by maintaining normal cytosolic Ca(2+) levels and INS secretion. May regulate beta cell mitochondrial Ca(2+) levels either indirectly via ER Ca(2+) efflux or directly by hyperpolarizing the mitochondrial membrane potential. Limits mitochondrial Ca(2+) oscillations and ATP production involved in glucose homeostasis upon metabolic stress. In pancreatic delta cells, limits Ca(2+)-induced Ca(2+)-release involved in somatostatin secretion and modulates islet paracrine signaling involved in glucagon secretion. Permeable to other monovalent cations such as Rb(+) and Cs(+). |
| Protein Name | Potassium Channel Subfamily K Member 162p Domain Potassium Channel Talk-1Twik-Related Alkaline Ph-Activated K(+ Channel 1Talk-1 |
| Database Links | Reactome: R-HSA-1299361Reactome: R-HSA-5576886 |
| Cellular Localisation | Isoform A: Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinCell MembraneIsoform B: Endoplasmic Reticulum MembraneMitochondrion Inner Membrane |
| Alternative Antibody Names | Anti-Potassium Channel Subfamily K Member 16 antibodyAnti-2p Domain Potassium Channel Talk-1 antibodyAnti-Twik-Related Alkaline Ph-Activated K(+ Channel 1 antibodyAnti-Talk-1 antibodyAnti-KCNK16 antibodyAnti-TALK1 antibody |
Information sourced from Uniprot.org