| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KCNA2 |
| Gene ID: |
3737 |
| Uniprot ID: |
KCNA2_HUMAN |
| Immunogen Region: |
1-165 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human KCNA2 (NP_004965.1). |
| Immunogen Sequence: |
MTVATGDPADEAAALPGHPQ DTYDPEADHECCERVVINIS GLRFETQLKTLAQFPETLLG DPKKRMRYFDPLRNEYFFDR NRPSFDAILYYYQSGGRLRR PVNVPLDIFSEEIRFYELGE EAMEMFREDEGYIKEEERPL PENEFQRQVWLLFEYPESSG PARII |
| Tissue Specificity | Detected in brain cortex. Detected in peroneal nerve in the juxtaparanodal regions of the node of Ranvier.expression is decreased in patients with diabetes mellitus that suffer from axonal neuropathy. Detected in paranodal and juxtanodal zones in myelinated spinal cord (at protein level). |
| Post Translational Modifications | Phosphorylated on tyrosine residues.phosphorylation increases in response to ischemia. Phosphorylated on tyrosine residues by activated PTK2B/PYK2. Phosphorylation on tyrosine residues suppresses ion channel activity. Phosphorylated on tyrosine residues in response to CHRM1 activation.this abolishes interaction with CTTN. This is probably due to endocytosis of the phosphorylated channel subunits. Phosphorylated on serine residues in response to increased cAMP levels.phosphorylation is apparently not catalyzed by PKA. N-glycosylated, with complex, sialylated N-glycans. |
| Function | Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain and the central nervous system, but also in the cardiovascular system. Prevents aberrant action potential firing and regulates neuronal output. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, KCNA6, KCNA7, and possibly other family members as well.channel properties depend on the type of alpha subunits that are part of the channel. Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation of delayed rectifier potassium channels. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Homotetrameric KCNA2 forms a delayed-rectifier potassium channel that opens in response to membrane depolarization, followed by slow spontaneous channel closure. In contrast, a heteromultimer formed by KCNA2 and KCNA4 shows rapid inactivation. Regulates neuronal excitability and plays a role as pacemaker in the regulation of neuronal action potentials. KCNA2-containing channels play a presynaptic role and prevent hyperexcitability and aberrant action potential firing. Response to toxins that are selective for KCNA2-containing potassium channels suggests that in Purkinje cells, dendritic subthreshold KCNA2-containing potassium channels prevent random spontaneous calcium spikes, suppressing dendritic hyperexcitability without hindering the generation of somatic action potentials, and thereby play an important role in motor coordination. Plays a role in the induction of long-term potentiation of neuron excitability in the CA3 layer of the hippocampus. May function as down-stream effector for G protein-coupled receptors and inhibit GABAergic inputs to basolateral amygdala neurons. May contribute to the regulation of neurotransmitter release, such as gamma-aminobutyric acid (GABA). Contributes to the regulation of the axonal release of the neurotransmitter dopamine. Reduced KCNA2 expression plays a role in the perception of neuropathic pain after peripheral nerve injury, but not acute pain. Plays a role in the regulation of the time spent in non-rapid eye movement (NREM) sleep. |
| Protein Name | Potassium Voltage-Gated Channel Subfamily A Member 2Ngk1Voltage-Gated K(+ Channel HukivVoltage-Gated Potassium Channel Hbk5Voltage-Gated Potassium Channel Subunit Kv1.2 |
| Database Links | Reactome: R-HSA-1296072 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinMembraneCell ProjectionAxonSynapseEndoplasmic Reticulum MembraneLamellipodium MembraneSynaptosomePresynaptic Cell MembraneDendriteCell JunctionParanodal Septate JunctionKcna2 By Itself Is Detected Both At The Endoplasmic Reticulum And At The Cell MembraneCoexpression With Kcna4 Or With Beta Subunits Promotes Expression At The Cell MembraneCoexpression With Kcna1 Inhibits Cell Surface ExpressionIn Myelinated Peripheral AxonsClustered In The Juxtaparadonal Region And At An Internodal Line Located Along The Mesaxon And Below The Schmidt-Lanterman Incisures |
| Alternative Antibody Names | Anti-Potassium Voltage-Gated Channel Subfamily A Member 2 antibodyAnti-Ngk1 antibodyAnti-Voltage-Gated K(+ Channel Hukiv antibodyAnti-Voltage-Gated Potassium Channel Hbk5 antibodyAnti-Voltage-Gated Potassium Channel Subunit Kv1.2 antibodyAnti-KCNA2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
