| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KAT2B (400-460 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
KAT2B |
| Gene ID: |
8850 |
| Uniprot ID: |
KAT2B_HUMAN |
| Immunogen Region: |
400-460 aa |
| Specificity: |
Specific for PCAF |
| Immunogen: |
A synthetic peptide from aa region 400-460 of human PCAF conjugated to an immunogenic carrier protein was used as the antigen. |
| Function | Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Has a a strong preference for acetylation of H3 at 'Lys-9' (H3K9ac). Also acetylates non-histone proteins, such as ACLY, MAPRE1/EB1, PLK4, RRP9/U3-55K and TBX5. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-BMAL1 and CLOCK-BMAL1 heterodimers. Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5. Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4. Acetylates RRP9/U3-55K, a core subunit of the U3 snoRNP complex, impairing pre-rRNA processing. Acetylates MAPRE1/EB1, promoting dynamic kinetochore-microtubule interactions in early mitosis. Also acetylates spermidine. (Microbial infection) In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. |
| Protein Name | Histone Acetyltransferase Kat2bHistone Acetyltransferase PcafHistone Acetylase PcafLysine Acetyltransferase 2bP300/Cbp-Associated FactorP/CafSpermidine Acetyltransferase Kat2b |
| Database Links | Reactome: R-HSA-1912408Reactome: R-HSA-2032785Reactome: R-HSA-210744Reactome: R-HSA-2122947Reactome: R-HSA-2644606Reactome: R-HSA-2894862Reactome: R-HSA-3214847Reactome: R-HSA-350054Reactome: R-HSA-5250924Reactome: R-HSA-5578768Reactome: R-HSA-5689901Reactome: R-HSA-73762Reactome: R-HSA-8936459Reactome: R-HSA-8941856Reactome: R-HSA-9013508Reactome: R-HSA-9013695Reactome: R-HSA-9018519Reactome: R-HSA-9617629Reactome: R-HSA-9772755Reactome: R-HSA-9793380 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeMainly Localizes To The NucleusAlso Localizes To Centrosomes In Late G1 And Around The G1/S TransitionCoinciding With The Onset Of Centriole FormationSubcellular Location May Vary Depending Upon Cell Differentiation StateCytoplasmic At The Very Stages Of Keratinocyte DifferentiationBecomes Nuclear At Later Differentiation StagesCytoplasmic In Basal Epithelial Cells (Undifferentiated Cells) And Nuclear In Parabasal Cells (Differentiated Cells)Localizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Histone Acetyltransferase Kat2b antibodyAnti-Histone Acetyltransferase Pcaf antibodyAnti-Histone Acetylase Pcaf antibodyAnti-Lysine Acetyltransferase 2b antibodyAnti-P300/Cbp-Associated Factor antibodyAnti-P/Caf antibodyAnti-Spermidine Acetyltransferase Kat2b antibodyAnti-KAT2B antibodyAnti-PCAF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
