• CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ Hep G2 cells with 1 Mu g JunD rabbit monoclonal antibody (STJ11102307) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of JunD in representative gene loci (TP53INP2) , as shown in figure.
  • Western blot analysis of extracts of various cell lines, using JunD rabbit monoclonal antibody (STJ11102307) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g JunD antibody (STJ11102307). Western blot was performed from the immunoprecipitate using JunD antibody (STJ11102307) at a dilution of 1:1000.
  • Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using JunD antibody (STJ11102307) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-JUND antibody (100-200) [S7MR] (STJ11102307)

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STJ11102307

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-JunD (100-200) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:500-1:1000
ChIP 1:500-1:1000
CUT&Tag 10⁵ cells/1 Mu g
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JUND
Gene ID: 3727
Uniprot ID: JUND_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human JunD (P17535).
Immunogen Sequence: SPELERLIIQSNGLVTTTPT SSQFLYPKVAASEEQEFAEG FVKALEDLHKQNQLGAGAAA AAAAAAAGGPSGTATGSAPP GELAPAAAAPEAPVYANLSS Y
Post Translational Modifications Phosphorylated by MAP kinases MAPK8 and MAPK10.phosphorylation is inhibited in the presence of MEN1.
Function Transcription factor binding AP-1 sites. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGAGCTCA-5' and enhancing their transcriptional activity.
Protein Name Transcription Factor Jund
Transcription Factor Ap-1 Subunit Jund
Database Links Reactome: R-HSA-9018519
Reactome: R-HSA-9031628
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Jund antibody
Anti-Transcription Factor Ap-1 Subunit Jund antibody
Anti-JUND antibody

Information sourced from Uniprot.org

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