• Western blot analysis of extracts of various cell lines, using JOSD1 antibody (STJ112458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-JOSD1 antibody (1-202) (STJ112458)

SKU:
STJ112458

$151.88 - $705.38
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-JOSD1 (1-202) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JOSD1
Gene ID: 9929
Uniprot ID: JOS1_HUMAN
Immunogen Region: 1-202
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-202 of human JOSD1 (NP_055691.1).
Immunogen Sequence: MSCVPWKGDKAKSESLELPQ AAPPQIYHEKQRRELCALHA LNNVFQDSNAFTRDTLQEIF QRLSPNTMVTPHKKSMLGNG NYDVNVIMAALQTKGYEAVW WDKRRDVGVIALTNVMGFIM NLPSSLCWGPLKLPLKRQHW ICVREVGGAYYNLDSKLKMP EWIGGESELRKFLKHHLRGK NCELLLVVPEEVEAHQSWRT DV
Post Translational Modifications Monoubiquitinated. Ubiquitination activates deubiquitination activity in vitro.
Function Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity.
Protein Name Josephin-1
Josephin Domain-Containing Protein 1
Database Links Reactome: R-HSA-5689877
Cellular Localisation Cell Membrane
Cytoplasm
Ubiquitination Increases Localization The Plasma Membrane
In The Cytosol
The Unubiquitinated Form May Be Associated With The Cytoskeleton Via Actb-Binding
Alternative Antibody Names Anti-Josephin-1 antibody
Anti-Josephin Domain-Containing Protein 1 antibody
Anti-JOSD1 antibody
Anti-JSPH1 antibody
Anti-KIAA0063 antibody

Information sourced from Uniprot.org

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