| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-JAK3 (350-530) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
JAK3 |
| Gene ID: |
3718 |
| Uniprot ID: |
JAK3_HUMAN |
| Immunogen Region: |
350-530 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 350-530 of JAK3 (NP_000206.2). |
| Immunogen Sequence: |
RLTTDSQHFFCKEVAPPRLL EEVAEQCHGPITLDFAINKL KTGGSRPGSYVLRRSPQDFD SFLLTVCVQNPLGPDYKGCL IRRSPTGTFLLVGLSRPHSS LRELLATCWDGGLHVDGVAV TLTSCCIPRPKEKSNLIVVQ RGHSPPTSSLVQPQSQYQLS QMTFHKIPADSLEWHENLGH G |
| Tissue Specificity | In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins. |
| Post Translational Modifications | Tyrosine phosphorylated in response to IL-2 and IL-4. Dephosphorylation of Tyr-980 and Tyr-981 by PTPN2 negatively regulates cytokine-mediated signaling (Probable). |
| Function | Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A and STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion. |
| Protein Name | Tyrosine-Protein Kinase Jak3Janus Kinase 3Jak-3Leukocyte Janus KinaseL-Jak |
| Database Links | Reactome: R-HSA-1266695Reactome: R-HSA-201556Reactome: R-HSA-5673001Reactome: R-HSA-6785807Reactome: R-HSA-8854691Reactome: R-HSA-8983432Reactome: R-HSA-8985947Reactome: R-HSA-9020558Reactome: R-HSA-9020958Reactome: R-HSA-912526Reactome: R-HSA-9679191 |
| Cellular Localisation | Endomembrane SystemPeripheral Membrane ProteinCytoplasm |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Jak3 antibodyAnti-Janus Kinase 3 antibodyAnti-Jak-3 antibodyAnti-Leukocyte Janus Kinase antibodyAnti-L-Jak antibodyAnti-JAK3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
