| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Intersectin-1 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ITSN1 |
| Gene ID: |
6453 |
| Uniprot ID: |
ITSN1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of Intersectin 1 at Human, Mouse, Rat |
| Immunogen: |
Synthesized peptide derived from human Intersectin 1 |
| Function | Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery. Acts as a guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex. Plays a role in the assembly and maturation of clathrin-coated vesicles. Recruits FCHSD2 to clathrin-coated pits. Involved in endocytosis of activated EGFR, and probably also other growth factor receptors. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR).internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines. Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones. Inhibits ARHGAP31 activity toward RAC1. Isoform 1: Plays a role in synaptic vesicle endocytosis in brain neurons. |
| Protein Name | Intersectin-1Sh3 Domain-Containing Protein 1aSh3p17 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-3928662Reactome: R-HSA-416482Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-9013148Reactome: R-HSA-9013406Reactome: R-HSA-9013408 |
| Cellular Localisation | Endomembrane SystemSynapseSynaptosomeCell ProjectionLamellipodiumCell MembraneMembraneClathrin-Coated PitRecycling EndosomeEndosomeCytoplasmic VesicleColocalizes With Sgip1 At The Plasma Membrane In Structures Corresponding Most Probably To Clathrin-Coated PitsColocalizes With Rab13 On Cytoplasmic Vesicles That Are Most Likely Recycling EndosomesIsoform 2: CytoplasmNucleus EnvelopeShuttles Between The Cytoplasm And Nucleus In An Xpo1/Crm1-Dependent MannerIsoform 5: Endomembrane System |
| Alternative Antibody Names | Anti-Intersectin-1 antibodyAnti-Sh3 Domain-Containing Protein 1a antibodyAnti-Sh3p17 antibodyAnti-ITSN1 antibodyAnti-ITSN antibodyAnti-SH3D1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
