| Host: |
Mouse |
| Applications: |
WB/ICC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Integrin Beta 4 (Cytoplasmic) is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M126 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHCICC 1:250IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ITGB4 |
| Gene ID: |
3691 |
| Uniprot ID: |
ITB4_HUMAN |
| Immunogen Region: |
Cytoplasmic |
| Specificity: |
This antibody detects a 200kDa* protein corresponding to the molecular mass of Integrin Beta 4 on SDS-PAGE immunoblots of human A431 cells. |
| Immunogen: |
Clone M126 was generated from a recombinant protein containing amino acid residues in the cytoplasmic region of human Integrin Beta 4. This sequence is found in all three Integrin Beta 4 isoforms and has 90% homology with rat and mouse Integrin Beta |
| Tissue Specificity | Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach. |
| Post Translational Modifications | Palmitoylated by DHHC3 at several cysteines of the membrane-proximal region, enhancing stability and cell surface expression. Palmitoylation also promotes secondary association with tertaspanins. |
| Function | Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling. |
| Protein Name | Integrin Beta-4Gp150Cd Antigen Cd104 |
| Database Links | Reactome: R-HSA-2022090Reactome: R-HSA-3000157Reactome: R-HSA-3000170Reactome: R-HSA-446107 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinLipid-AnchorCell JunctionHemidesmosomeColocalizes With Dst At The Leading Edge Of Migrating Keratinocytes |
| Alternative Antibody Names | Anti-Integrin Beta-4 antibodyAnti-Gp150 antibodyAnti-Cd Antigen Cd104 antibodyAnti-ITGB4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
