• Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ITGB1BP1 antibody (STJ191876)

SKU:
STJ191876

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Integrin beta-1-binding protein 1 is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGB1BP1
Gene ID: 9270
Uniprot ID: ITBP1_HUMAN
Specificity: ITBP1 Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from part of the human protein
Post Translational Modifications Phosphorylation at Thr-38 seems to enhance integrin alpha5beta1-mediated cell adhesion. The degree of phosphorylation is regulated by integrin-dependent cell-matrix interaction.
Function Key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail and preventing the activation of integrin alpha-5/beta-1 (heterodimer of ITGA5 and ITGB1) by talin or FERMT1. Plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. Stimulates cellular proliferation in a fibronectin-dependent manner. Involved in the regulation of beta-1 integrin-containing focal adhesion (FA) site dynamics by controlling its assembly rate during cell adhesion.inhibits beta-1 integrin clustering within FA by directly competing with talin TLN1, and hence stimulates osteoblast spreading and migration in a fibronectin- and/or collagen-dependent manner. Acts as a guanine nucleotide dissociation inhibitor (GDI) by regulating Rho family GTPases during integrin-mediated cell matrix adhesion.reduces the level of active GTP-bound form of both CDC42 and RAC1 GTPases upon cell adhesion to fibronectin. Stimulates the release of active CDC42 from the membranes to maintain it in an inactive cytoplasmic pool. Participates in the translocation of the Rho-associated protein kinase ROCK1 to membrane ruffles at cell leading edges of the cell membrane, leading to an increase of myoblast cell migration on laminin. Plays a role in bone mineralization at a late stage of osteoblast differentiation.modulates the dynamic formation of focal adhesions into fibrillar adhesions, which are adhesive structures responsible for fibronectin deposition and fibrillogenesis. Plays a role in blood vessel development.acts as a negative regulator of angiogenesis by attenuating endothelial cell proliferation and migration, lumen formation and sprouting angiogenesis by promoting AKT phosphorylation and inhibiting ERK1/2 phosphorylation through activation of the Notch signaling pathway. Promotes transcriptional activity of the MYC promoter.
Protein Name Integrin Beta-1-Binding Protein 1
Integrin Cytoplasmic Domain-Associated Protein 1
Icap-1
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Cell Membrane
Cell Projection
Lamellipodium
Ruffle
Nucleocytoplasmic Shuttling Protein
Shuttles Between Nucleus And Cytoplasm In A Integrin-Dependent Manner
Probably Sequestered In The Cytosol By Itgb1
Its Localization Is Dependent On The Stage Of Cell Spreading On Fibronectin
Cytoplasmic In Case Of Round Cells
Corresponding To The Initial Step Of Cell Spreading
Or Nuclear In Case Of Well Spread Cells
Colocalizes With Rock1 And Nme2 At Beta-1 Integrin Engagement Sites
Together With Itgb1 And Nme2 Is Recruited To Beta-1 Integrin-Rich Peripheral Ruffles And Lamellipodia During Initial Cell Spreading On Fibronectin And/Or Collagen
Alternative Antibody Names Anti-Integrin Beta-1-Binding Protein 1 antibody
Anti-Integrin Cytoplasmic Domain-Associated Protein 1 antibody
Anti-Icap-1 antibody
Anti-ITGB1BP1 antibody
Anti-ICAP1 antibody

Information sourced from Uniprot.org

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