| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-E3 Ubiquitin-Protein Ligase Itchy Homolog (1-155) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ITCH |
| Gene ID: |
83737 |
| Uniprot ID: |
ITCH_HUMAN |
| Immunogen Region: |
1-155 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-155 of ITCH (NP_001244066.1). |
| Immunogen Sequence: |
MSDSGSQLGSMGSLTMKSQL QITVISAKLKENKKNWFGPS PYVEVTVDGQSKKTEKCNNT NSPKWKQPLTVIVTPVSKLH FRVWSHQTLKSDVLLGTAAL DIYETLKSNNMKLEEVVVTL QLGGDKEPTETIGDLSICLD GLQLESEVVTNGETT |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | On T-cell activation, phosphorylation by the JNK cascade on serine and threonine residues surrounding the PRR domain accelerates the ubiquitination and degradation of JUN and JUNB. The increased ITCH catalytic activity due to phosphorylation by JNK1 may occur due to a conformational change disrupting the interaction between the PRR/WW motifs domain and the HECT domain and, thus exposing the HECT domain. Phosphorylation by FYN reduces interaction with JUNB and negatively controls JUN ubiquitination and degradation. Monoubiquitinated. Autopolyubiquitinated with 'Lys-63' linkages which does not lead to protein degradation. |
| Function | Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. Involved in the control of inflammatory signaling pathways. Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages. Mediates JUN ubiquitination and degradation. Mediates JUNB ubiquitination and degradation. Critical regulator of type 2 helper T (Th2) cell cytokine production by inducing JUNB ubiquitination and degradation. Involved in the negative regulation of MAVS-dependent cellular antiviral responses. Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteasomal degradation. Following ligand stimulation, regulates sorting of Wnt receptor FZD4 to the degradative endocytic pathway probably by modulating PI42KA activity. Ubiquitinates PI4K2A and negatively regulates its catalytic activity. Ubiquitinates chemokine receptor CXCR4 and regulates sorting of CXCR4 to the degradative endocytic pathway following ligand stimulation by ubiquitinating endosomal sorting complex required for transport ESCRT-0 components HGS and STAM. Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination. Ubiquitinates SNX9. Ubiquitinates MAP3K7 through 'Lys-48'-linked conjugation. Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP. Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. Inhibits the replication of influenza A virus (IAV) via ubiquitination of IAV matrix protein 1 (M1) through 'Lys-48'-linked conjugation resulting in M1 proteasomal degradation. Ubiquitinates NEDD9/HEF1, resulting in proteasomal degradation of NEDD9/HEF1. |
| Protein Name | E3 Ubiquitin-Protein Ligase Itchy HomologItchAtrophin-1-Interacting Protein 4Aip4Hect-Type E3 Ubiquitin Transferase Itchy HomologNfe2-Associated Polypeptide 1Napp1 |
| Database Links | Reactome: R-HSA-1253288Reactome: R-HSA-168638Reactome: R-HSA-2122948Reactome: R-HSA-5610780Reactome: R-HSA-5632684Reactome: R-HSA-5675482Reactome: R-HSA-8939236Reactome: R-HSA-936440Reactome: R-HSA-9692916Reactome: R-HSA-983168 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmNucleusEarly Endosome MembraneEndosome MembraneMay Be Recruited To Exosomes By Ndfip1Localizes To Plasma Membrane Upon Cxcl12 Stimulation Where It Co-Localizes With Cxcl4Localization To Early Endosomes Is Increased Upon Cxcl12 Stimulation Where It Co-Localizes With Dtx3l And Cxcl4 |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Itchy Homolog antibodyAnti-Itch antibodyAnti-Atrophin-1-Interacting Protein 4 antibodyAnti-Aip4 antibodyAnti-Hect-Type E3 Ubiquitin Transferase Itchy Homolog antibodyAnti-Nfe2-Associated Polypeptide 1 antibodyAnti-Napp1 antibodyAnti-ITCH antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and ITCH knockout (KO) 293T cells, using [KO Validated] ITCH Rabbit polyclonal antibody (STJ118061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/96951/379801/STJ118061_1__34949.1713147667.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ITCH knockout (KO) 293T cells, using [KO Validated] ITCH Rabbit polyclonal antibody (STJ118061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
