| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-IRF5 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 55+60kDa bands observed in Human Spleen lysates and approx 65kDa in lysates of cell line A549 (calculated MW of 57.8kDa according to NP_001092099.1 and 56.0kDa according to NP_ |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | IRF5 |
| Gene ID: | 3663 |
| Uniprot ID: | IRF5_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_116032.1; NP_001092097.2; NP_001092099.1 |
| Specificity: | This antibody is expected to recognize reported isoforms (NP_116032.1; NP_001092097.2 ; NP_001092099.1 |
| Immunogen Sequence: | QGPWPMHPAGMQ |
| Function | Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway. Key transcription factor regulating the IFN response during SARS-CoV-2 infection. |
| Protein Name | Interferon Regulatory Factor 5Irf-5 |
| Database Links | Reactome: R-HSA-877300Reactome: R-HSA-909733Reactome: R-HSA-9860276 |
| Cellular Localisation | CytoplasmNucleusShuttles Between The Nucleus And The Cytoplasm: Upon Activation By The Tlr Adapter Myd88 And Subsequent PhosphorylationTranslocates To The Nucleus |
| Alternative Antibody Names | Anti-Interferon Regulatory Factor 5 antibodyAnti-Irf-5 antibodyAnti-IRF5 antibody |
Information sourced from Uniprot.org