• Western blot analysis of various lysates using IRAK3 Rabbit polyclonal antibody (STJ115364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IRAK3 antibody (367-596) (STJ115364)

SKU:
STJ115364

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IRAK3 (367-596) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IRAK3
Gene ID: 11213
Uniprot ID: IRAK3_HUMAN
Immunogen Region: 367-596
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 367-596 of human IRAK3 (NP_009130.2).
Immunogen Sequence: VLTGCRVVLDDPKHIQLRDL LRELMEKRGLDSCLSFLDKK VPPCPRNFSAKLFCLAGRCA ATRAKLRPSMDEVLNTLEST QASLYFAEDPPTSLKSFRCP SPLFLENVPSIPVEDDESQN NNLLPSDEGLRIDRMTQKTP FECSQSEVMFLSLDKKPESK RNEEACNMPSSSCEESWFPK YIVPSQDLRPYKVNIDPSSE APGHSCRSRPVESSCSSKFS WDEYEQYKKE
Tissue Specificity Expressed in eosinophils, dendritic cells and/or monocytes (at protein level). Expressed predominantly in peripheral blood lymphocytes.
Function Putative inactive protein kinase which regulates signaling downstream of immune receptors including IL1R and Toll-like receptors. Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex. Upon IL33-induced lung inflammation, positively regulates expression of IL6, CSF3, CXCL2 and CCL5 mRNAs in dendritic cells.
Protein Name Interleukin-1 Receptor-Associated Kinase 3
Irak-3
Il-1 Receptor-Associated Kinase M
Irak-M
Inactive Il-1 Receptor-Associated Kinase 3
Database Links Reactome: R-HSA-166058
Reactome: R-HSA-9020702
Cellular Localisation Cytoplasm
Nucleus
In Dendritic Cells
Translocates Into The Nucleus Upon Il33 Stimulation
Alternative Antibody Names Anti-Interleukin-1 Receptor-Associated Kinase 3 antibody
Anti-Irak-3 antibody
Anti-Il-1 Receptor-Associated Kinase M antibody
Anti-Irak-M antibody
Anti-Inactive Il-1 Receptor-Associated Kinase 3 antibody
Anti-IRAK3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance