Anti-IRAK-4 antibody [2H9] (STJ98182)

Anti-IRAK-4 antibody [2H9] (STJ98182)

SKU:
STJ98182
£149.00 - £360.00
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Short Description :Mouse monoclonal anti-Interleukin-1 receptor-associated kinase 4 for use in WB, IHC, IF, FC and ELISA in Human, Mouse and Monkey samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/IHC/IF/FC/ELISA
Host:Mouse
Reactivity:Human/Mouse/Monkey
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Monoclonal
Clone ID :2H9
Isotype:IgG1
Conjugation:Unconjugated
Purification:Affinity purification
Dilution Range:WB 1:500-1:2000
IHC 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
IF 1:50-200
Formulation:Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:IRAK4
Gene ID:51135
Uniprot ID:IRAK4_HUMAN
Immunogen:Purified recombinant fragment of human IRAK-4 expressed in E. Coli.
Specificity:IRAK-4 Monoclonal Antibody detects endogenous levels of IRAK-4 protein.
Function Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
Protein Name Interleukin-1 Receptor-Associated Kinase 4
Irak-4
Renal Carcinoma Antigen Ny-Ren-64
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-166058
Reactome: R-HSA-5603037
Reactome: R-HSA-5603041
Reactome: R-HSA-6811558
Reactome: R-HSA-9020702
Reactome: R-HSA-975110
Reactome: R-HSA-975138
Reactome: R-HSA-975155
Reactome: R-HSA-975871
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Interleukin-1 Receptor-Associated Kinase 4 antibody
Anti-Irak-4 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-64 antibody
Anti-IRAK4 antibody

Information sourced from Uniprot.org

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