• Anti-IRAK-4 antibody [2H9] (STJ98182)
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer tissues with DAB staining using IRAK-4 monoclonal antibody.
  • Flow cytometric analysis of Hela cells using IRAK-4 monoclonal antibody (blue) and negative control (red).
  • Western blot analysis using IRAK-4 monoclonal antibody against THP-1 (1) , HeLa (2) , K562 (3) , MCF-7 (4) , RAW264.7 (5) , Jurkat (6) and Cos7 (7) cell lysate.

Anti-IRAK-4 antibody [2H9] (STJ98182)


Current Stock:
Host: Mouse
Applications: WB, IHC-P, FC, ELISA
Reactivity: Human, Mouse, Monkey
Short Description: Mouse monoclonal anti-IRAK-4 antibody is suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and ELISA.
Clonality: Monoclonal
Clone ID: 2H9
Conjugation: Unconjugated
Formulation: Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.
Purification: Affinity purification
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/mL
Isotype: IgG1
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Specificity: IRAK-4 monoclonal antibody detects endogenous levels of IRAK-4 protein.
Gene ID: 51135
Gene Symbol: IRAK4
Immunogen: Purified recombinant fragment of human IRAK-4 expressed in E. Coli.
Function Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
Protein Name Interleukin-1 Receptor-Associated Kinase 4
Renal Carcinoma Antigen Ny-Ren-64
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-166058
Reactome: R-HSA-5603037
Reactome: R-HSA-5603041
Reactome: R-HSA-6811558
Reactome: R-HSA-9020702
Reactome: R-HSA-975110
Reactome: R-HSA-975138
Reactome: R-HSA-975155
Reactome: R-HSA-975871
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Interleukin-1 Receptor-Associated Kinase 4 antibody
Anti-Irak-4 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-64 antibody
Anti-IRAK4 antibody

Information sourced from Uniprot.org

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