![Western blot analysis of lysates from wild type (WT) and IPO4 knockout (KO) 293T cells, using [KO Validated] IPO4 Rabbit polyclonal antibody (STJ11100778) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89714/358196/STJ11100778_1__20770.1713122514.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and IPO4 knockout (KO) 293T cells, using [KO Validated] IPO4 Rabbit polyclonal antibody (STJ11100778) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and IPO4 knockout (KO) 293T cells, using [KO Validated] IPO4 Rabbit polyclonal antibody (STJ11100778) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89714/358196/STJ11100778_1__20770.1713122514.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and IPO4 knockout (KO) 293T cells, using [KO Validated] IPO4 Rabbit polyclonal antibody (STJ11100778) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-IPO4 antibody (728-1081) (STJ11100778)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal IPO4 (728-1081) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IPO4 |
| Gene ID | 79711 |
| Uniprot ID | IPO4_HUMAN |
| Immunogen Region | 728-1081 |
| Immunogen Sequence | LHKACQSCPSEPNTAALQAA LARVVPSYMQAVNRERERQV VMAVLEALTGVLRSCGTLTL KPPGRLAELCGVLKAVLQRK TACQDTDEEEEEEDDDQAEY DAMLLEHAGEAIPALAAAAG GDSFAPFFAGFLPLLVCKTK QGCTVAEKSFAVGTLAETIQ GLGAASAQFVSRLLPVLLST AQEADPEVRSNAIFGMGVLA EHGGHPAQEHFPKLLGLLFP LLARERHDRVRDNICGALA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 728-1081 of human IPO4 (NP_078934.3). |
Additional Info
| Function | Nuclear transport receptor that mediates nuclear import of proteins, such as histones, RPS3A, TNP2 and VDR. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of the histone H3-H4 dimer when in complex with ASF1 (ASF1A or ASF1B). Mediates the ligand-independent nuclear import of vitamin D receptor (VDR). In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. |
| Protein Name | Importin-4Imp4Importin-4bImp4bRan-Binding Protein 4Ranbp4 |
| Database Links | |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Importin-4 antibodyAnti-Imp4 antibodyAnti-Importin-4b antibodyAnti-Imp4b antibodyAnti-Ran-Binding Protein 4 antibodyAnti-Ranbp4 antibodyAnti-IPO4 antibodyAnti-IMP4B antibodyAnti-RANBP4 antibody |
Information sourced from Uniprot.org