Anti-INTS8 antibody (600-800) (STJ11103355)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal INTS8 (600-800) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | INTS8 |
| Gene ID | 55656 |
| Uniprot ID | INT8_HUMAN |
| Immunogen Region | 600-800 |
| Immunogen Sequence | VTEFSPKLRQVMLNEMLLLD IHTHEAGTGQAGERPPSDLI SRVRGYLEMRLPDIPLRQVI AEECVAFMLNWRENEYLTLQ VPAFLLQSNPYVKLGQLLAA TCKELPGPKESRRTAKDLWE VVVQICSVSSQHKRGNDGRV SLIKQRESTLGIMYRSELLS FIKKLREPLVLTIILSLFVK LHNVREDIVNDITAEHISIW P |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 600-800 of human INTS8 (NP_060334.2). |
Additional Info
| Function | Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes. The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA. The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs). Within the integrator complex, INTS8 is required for the recruitment of protein phosphatase 2A (PP2A) to transcription pause-release checkpoint. |
| Protein Name | Integrator Complex Subunit 8Int8Protein Kaonashi-1 |
| Database Links | Reactome: R-HSA-6807505 |
| Cellular Localisation | NucleusChromosomeAssociates With Chromatin And Transcription Pause-Release Checkpoint |
| Alternative Antibody Names | Anti-Integrator Complex Subunit 8 antibodyAnti-Int8 antibodyAnti-Protein Kaonashi-1 antibodyAnti-INTS8 antibodyAnti-C8orf52 antibody |
Information sourced from Uniprot.org