| Host: |
Mouse |
| Applications: |
ELISA/FC/WB/IHC/IP |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Interferon Gamma is suitable for use in ELISA, Flow Cytometry, Western Blot, Immunohistochemistry and Immunoprecipitation research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
G30 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2bk |
| Formulation: |
PBS with 0.02% Sodium Azide. |
| Purification: |
Affinity purified from tissue culture. |
| Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: |
ELISA (solid phase: 0, 1-100 µg/ml; tracer: 0, 001-100 µg/ml for 30 min at RT). Flow cytometry (0.5-1.0 µg/million cells in 0.1 ml). Immunoblotting (1-2 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; an appropriate antigen retrieval method for |
| Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: |
IFNG |
| Gene ID: |
3458 |
| Uniprot ID: |
IFNG_HUMAN |
| Immunogen: |
A BALB/c mouse was immunized with E.coli derived recombinant human IFN Gamma (aa22-166).Fusion partner: NS-0. |
| Tissue Specificity | Released primarily from activated T lymphocytes. |
| Post Translational Modifications | Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. |
| Function | Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation. |
| Protein Name | Interferon GammaIfn-GammaImmune Interferon |
| Database Links | Reactome: R-HSA-877300Reactome: R-HSA-877312Reactome: R-HSA-8877330Reactome: R-HSA-8950505Reactome: R-HSA-9732724 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Interferon Gamma antibodyAnti-Ifn-Gamma antibodyAnti-Immune Interferon antibodyAnti-IFNG antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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