• Immunofluorescence analysis of PC-12 cells using INPP5K Rabbit polyclonal antibody (STJ110117) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using INPP5K Rabbit polyclonal antibody (STJ110117) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using INPP5K Rabbit polyclonal antibody (STJ110117) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-549 cells using INPP5K Rabbit polyclonal antibody (STJ110117) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse skeletal muscle, using INPP5K Rabbit polyclonal antibody (STJ110117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from 293T cells, using INPP5K Rabbit polyclonal antibody (STJ110117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-INPP5K antibody (149-448) (STJ110117)

SKU:
STJ110117

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-INPP5K (149-448) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INPP5K
Gene ID: 51763
Uniprot ID: INP5K_HUMAN
Immunogen Region: 149-448
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 149-448 of human INPP5K (NP_057616.2).
Immunogen Sequence: HLPPHISNNYQRLEHFDRIL EMQNCEGRDIPNILDHDLII WFGDMNFRIEDFGLHFVRES IKNRCYGGLWEKDQLSIAKK HDPLLREFQEGRLLFPPTYK FDRNSNDYDTSEKKRKPAWT DRILWRLKRQPCAGPDTPIP PASHFSLSLRGYSSHMTYGI SDHKPVSGTFDLELKPLVSA PLIVLMPEDLWTVENDMMVS YSSTSDFPSSPWDWIGLYKV GLRDVNDYVSYAWVGDSKV
Tissue Specificity Ubiquitously expressed with highest levels in skeletal muscle, heart and kidney.
Function Inositol 5-phosphatase which acts on inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Has 6-fold higher affinity for phosphatidylinositol 4,5-bisphosphate than for inositol 1,4,5-trisphosphate. Negatively regulates assembly of the actin cytoskeleton. Controls insulin-dependent glucose uptake among inositol 3,4,5-trisphosphate phosphatases.therefore, is the specific regulator for insulin signaling in skeletal muscle.
Protein Name Inositol Polyphosphate 5-Phosphatase K
Phosphatidylinositol-3 -4 -5-Trisphosphate 5-Phosphatase
Phosphatidylinositol-4 -5-Bisphosphate 5-Phosphatase
Skeletal Muscle And Kidney-Enriched Inositol Phosphatase
Database Links Reactome: R-HSA-1660499
Cellular Localisation Endoplasmic Reticulum
Cytoplasm
Following Stimulation With Egf
Translocates To Membrane Ruffles
Concentrated At The Periphery Of The Nucleus
Alternative Antibody Names Anti-Inositol Polyphosphate 5-Phosphatase K antibody
Anti-Phosphatidylinositol-3 -4 -5-Trisphosphate 5-Phosphatase antibody
Anti-Phosphatidylinositol-4 -5-Bisphosphate 5-Phosphatase antibody
Anti-Skeletal Muscle And Kidney-Enriched Inositol Phosphatase antibody
Anti-INPP5K antibody
Anti-PPS antibody
Anti-SKIP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance