• Western blot analysis of extracts of various cell lines, using INPP4A antibody (STJ110004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-INPP4A antibody (1-250) (STJ110004)

SKU:
STJ110004

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INPP4A
Gene ID: 3631
Uniprot ID: INP4A_HUMAN
Immunogen Region: 1-250
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human INPP4A (NP_004018.1).
Immunogen Sequence: MTAREHSPRHGARARAMQRA STIDVAADMLGLSLAGNIQD PDEPILEFSLACSELHTPSL DRKPNSFVAVSVTTPPQAFW TKHAQTEIIEGTNNPIFLSS IAFFQDSLINQMTQVKLSVY DVKDRSQGTMYLLGSGTFIV KDLLQDRHHRLHLTLRSAES DRVGNITVIGWQMEEKSDQR PPVTRSVDTVNGRMVLPVDE SLTEALGIRSKYASLRKDTL LKSVFGGAICRMYRFPTTD
Tissue Specificity Isoform 1 is expressed in the platelets, MEG-01 megakaryocytes and Jurkat T-cells. Isoform 2 is expressed in the brain.
Function Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2). Catalyzes also inositol 1,3,4-trisphosphate and inositol 1,4-bisphosphate. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. May protect neurons from excitotoxic cell death by regulating the synaptic localization of cell surface N-methyl-D-aspartate-type glutamate receptors (NMDARs) and NMDAR-mediated excitatory postsynaptic current. Isoform 4: Displays no 4-phosphatase activity for PtdIns(3,4)P2, Ins(3,4)P2, or Ins(1,3,4)P3.
Protein Name Inositol Polyphosphate-4-Phosphatase Type I A
Inositol Polyphosphate 4-Phosphatase Type I
Type I Inositol 3 -4-Bisphosphate 4-Phosphatase
Database Links Reactome: R-HSA-1660499
Reactome: R-HSA-1660516
Reactome: R-HSA-1855183
Cellular Localisation Early Endosome Membrane
Recycling Endosome Membrane
Cell Membrane
Nucleus
Cytoplasm
Postsynaptic Density
Translocates To The Plasma Membrane Upon Egf Stimulation
Shuttles Between The Cytoplasm And The Nucleus
Depending On The Cell Cycle Stage
With Highest Amounts Detected In The Nucleus During The G0/G1phase
Alternative Antibody Names Anti-Inositol Polyphosphate-4-Phosphatase Type I A antibody
Anti-Inositol Polyphosphate 4-Phosphatase Type I antibody
Anti-Type I Inositol 3 -4-Bisphosphate 4-Phosphatase antibody
Anti-INPP4A antibody

Information sourced from Uniprot.org

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