• Confocal imaging of RAW264. 7 LPS and RAW264. 7 cells using iNOS Rabbit monoclonal antibody (STJ11103535, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of lysates from RAW264. 7 cells, using iNOS Rabbit monoclonal antibody (STJ11103535) at 1:1000 dilution. RAW264. 7 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-iNOS antibody (2-200) [S5MR] (STJ11103535)

SKU:
STJ11103535

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-iNOS (2-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOS2
Gene ID: 4843
Uniprot ID: NOS2_HUMAN
Immunogen Region: 2-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2-200 of human iNOS (P35228).
Immunogen Sequence: ACPWKFLFKTKFHQYAMNGE KDINNNVEKAPCATSSPVTQ DDLQYHNLSKQQNESPQPLV ETGKKSPESLVKLDATPLSS PRHVRIKNWGSGMTFQDTLH HKAKGILTCRSKSCLGSIMT PKSLTRGPRDKPTPPDELLP QAIEFVNQYYGSFKEAKIEE HLARVEAVTKEIETTGTYQL TGDELIFATKQAWRNAPRC
Tissue Specificity Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Expressed in chondrocytes.
Post Translational Modifications Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation.
Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8.
Protein Name Nitric Oxide Synthase - Inducible
Hepatocyte Nos
Hep-Nos
Inducible No Synthase
Inducible Nos
Inos
Nos Type Ii
Peptidyl-Cysteine S-Nitrosylase Nos2
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-392154
Reactome: R-HSA-6785807
Reactome: R-HSA-9033241
Reactome: R-HSA-9636249
Cellular Localisation Cytoplasm
Cytosol
Localizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4
Exhibits A More Diffuse Cytosolic Localization
Alternative Antibody Names Anti-Nitric Oxide Synthase - Inducible antibody
Anti-Hepatocyte Nos antibody
Anti-Hep-Nos antibody
Anti-Inducible No Synthase antibody
Anti-Inducible Nos antibody
Anti-Inos antibody
Anti-Nos Type Ii antibody
Anti-Peptidyl-Cysteine S-Nitrosylase Nos2 antibody
Anti-NOS2 antibody
Anti-NOS2A antibody

Information sourced from Uniprot.org

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